CAJ | 학술논문

目的：寻找糖尿病肾病敏感的血浆分子标志物。方法对8例早期糖尿病肾病患者的血浆和正常血浆进行蛋白质组学荧光差异双向电泳（2-D DIGE）分析，选择表达差异大于1．5倍的蛋白斑点进行质谱分析。结果成功建立糖尿病肾病和正常血浆的胶内差异双向凝胶电泳图谱，通过分析共鉴定出13种差异蛋白质，包括C3补体、C4补体、载脂蛋白E等。结论2-D DIGE能够客观地显示糖尿病肾病与正常血浆之间的蛋白质表达差异，本研究鉴定的13种蛋白有可能为糖尿病肾病的早期诊断提供潜在的血浆分子标志物。
목적：심조당뇨병신병민감적혈장분자표지물。방법대8례조기당뇨병신병환자적혈장화정상혈장진행단백질조학형광차이쌍향전영（2-D DIGE）분석，선택표체차이대우1．5배적단백반점진행질보분석。결과성공건립당뇨병신병화정상혈장적효내차이쌍향응효전영도보，통과분석공감정출13충차이단백질，포괄C3보체、C4보체、재지단백E등。결론2-D DIGE능구객관지현시당뇨병신병여정상혈장지간적단백질표체차이，본연구감정적13충단백유가능위당뇨병신병적조기진단제공잠재적혈장분자표지물。
Objective To explore the sensitive blood plasma molecular markers in diabetic nephropathy (DN) .Methods Two-di-mensional fluorescence difference gel electrophoresis (2D-DIGE) was used to analyze early DN patients plasma (n=8) and normal plasma ,proteins that showed differential expression of a 1 .5 fold change were analyzed by MALDI-TOF/TOF mass spectrometry . Results 2D-DIGE maps of plasma proteins in patients with DN and normal plasma were established successfully .We validated 13 differentially expressed proteins detected by 2D-DIGE ,including complement component C3、complement component C4、apolipopro-tein E ,etc .Conclusion Proteomic analysis can objectively revealed the differences of protein expression between the two kinds of blood plasma .The 13 proteins might be the potential plasma molecular markers for the clinical diagnosis of diabetic nephropathy .