CAJ | 학술논문

目的：观察艾叶水提取物对H27人肝癌细胞抑制作用及对Bcl-2、热休克蛋白（HSP70）蛋白表达的影响。方法从细胞的形态学观察，并进一步使用免疫细胞化学剂、蛋白免疫印迹法（Westernblotting）等分析方法研究癌基因Bcl-2及HSP70蛋白的表达情况。结果60mg/mL的艾叶水提取物处理肝癌细胞12h后，肝癌细胞均出现严重皱缩、固缩、细胞质膜突出等，免疫组化及Westernblotting分析显示实验过程中伴随着癌基因Bcl-2表达的减弱（P＜0．05），而HSP70蛋白表达则无明显变化（P＞0．05）。结论艾叶水提取物能够诱导体外人肝癌细胞凋亡，而这个凋亡过程可能跟癌基因Bcl-2有关，与HSP70的表达关系不明显。
목적：관찰애협수제취물대H27인간암세포억제작용급대Bcl-2、열휴극단백（HSP70）단백표체적영향。방법종세포적형태학관찰，병진일보사용면역세포화학제、단백면역인적법（Westernblotting）등분석방법연구암기인Bcl-2급HSP70단백적표체정황。결과60mg/mL적애협수제취물처리간암세포12h후，간암세포균출현엄중추축、고축、세포질막돌출등，면역조화급Westernblotting분석현시실험과정중반수착암기인Bcl-2표체적감약（P＜0．05），이HSP70단백표체칙무명현변화（P＞0．05）。결론애협수제취물능구유도체외인간암세포조망，이저개조망과정가능근암기인Bcl-2유관，여HSP70적표체관계불명현。
Objective To explore the leaves water extract on the liver human hepatocellular carcinoma xenografts and protein expression of Bcl-2 and HSP70 .Methods From cell morphology and DNA agarose gel electrophoresis the observation leaves water extract the characteristics of the material role in human hepatoma cells ,further use of immunocytochemistry agent Western blotting analysis method oncogene Bcl-2 and tumor suppressor genes HSP70expression .Results After the processing of the the 60 mg/mL leaves water extract of liver cancer cells after 12 h ,liver cells showed severe shrinkage ,pyknosis ,highlight the cell plasma mem-brane ,immunohistochemistry and Western blotting analysis showed that during the experiment along with the oncogene Bcl-2 ex-pression weakened(P<0 .05) ,while the expression of tumor suppressor genes HSP70 had no obvious change(P<0 .05) .Conclusion Water extract of the leaves can be induced in vitro human liver cancer cell apoptosis ,and the apoptotic process may be followed by the oncogene Bcl-2 ,tumor suppressor gene expression of HSP70 relationship .