CAJ | 학술논문

目的：研究蛋白酶激活受体-1（PAR-1）活化后对小鼠内皮祖细胞（EPCs） CXC型趋化因子受体4（CXCR4） mRNA表达及细胞增殖、迁移的影响。方法分离鉴定小鼠 EPCs ，分别以不同浓度 PAR-1激活剂SFLLRN加入培养的EPCs中，以PAR-1小干扰RNA（siRNA）转染EPCs ，并设立空白对照组。采用荧光定量实时聚合酶链反应（RT-PCR）分析各组细胞PAR-1、CXCR4 mRNA表达；MTT法检测各组EPCs增殖情况；Transwell小室共培养分析各组EPCs的迁移能力。结果 SFLLRN激活组EPCs增殖率、迁移率及PAR-1、CXCR4 mRNA表达均较空白对照组、PAR-1特异siRNA转染组增高，差异有统计学意义（ P＜0．05）。CXCR4 mRNA与PAR-1 mRNA表达呈明显正相关（r＝0．991）。结论 PAR-1可能通过促使CXCR4的表达增加，从而促进EPCs的增殖和迁移。
목적：연구단백매격활수체-1（PAR-1）활화후대소서내피조세포（EPCs） CXC형추화인자수체4（CXCR4） mRNA표체급세포증식、천이적영향。방법분리감정소서 EPCs ，분별이불동농도 PAR-1격활제SFLLRN가입배양적EPCs중，이PAR-1소간우RNA（siRNA）전염EPCs ，병설립공백대조조。채용형광정량실시취합매련반응（RT-PCR）분석각조세포PAR-1、CXCR4 mRNA표체；MTT법검측각조EPCs증식정황；Transwell소실공배양분석각조EPCs적천이능력。결과 SFLLRN격활조EPCs증식솔、천이솔급PAR-1、CXCR4 mRNA표체균교공백대조조、PAR-1특이siRNA전염조증고，차이유통계학의의（ P＜0．05）。CXCR4 mRNA여PAR-1 mRNA표체정명현정상관（r＝0．991）。결론 PAR-1가능통과촉사CXCR4적표체증가，종이촉진EPCs적증식화천이。
Objective To explore the effect of protease-activated receptor 1(PAR-1) activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells (EPCs) and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN (one agonist of PAR-1) ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups(P< 0 .05) .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA(r=0 .991) .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .