CAJ | 학술논문

目的：探讨坐骨神经旁注射右美托咪啶对福尔马林致痛小鼠痛行为学及脊髓c-fos表达的影响。方法雄性成年昆明小鼠32只，体质量20～25 g ，随机分为4组，每组8只。A组在小鼠右侧坐骨神经旁注射生理盐水；B组在小鼠右足底皮下注射5％福尔马林（10μL ），建立急性炎性痛模型；C组在小鼠致痛前15 min预先在右侧坐骨神经旁注射右美托咪啶0．1μg ；D组在小鼠右侧坐骨神经旁注射右美托咪啶0．1μg。观察各组小鼠Ⅰ、Ⅱ时相舔咬足时间，在2 h时间点处死小鼠，取腰L4～5段脊髓，行免疫荧光检测c-fos的表达。结果与A组比较，B组小鼠在Ⅰ、Ⅱ时相出现明显的舔咬足行为，疼痛评分明显增高，脊髓c-fos的表达明显增多（P＜0．05）；与B组比较，C组舔咬足时间明显缩短，疼痛评分明显降低、脊髓c-fos的表达明显减少（P＜0．05）；A、C、D组疼痛评分和脊髓c-fos的表达差异无统计学意义。结论坐骨神经旁注射右美托咪啶能够减轻小鼠足底注射福尔马林引起的急性炎性痛，并且降低脊髓c-fos的表达。
목적：탐토좌골신경방주사우미탁미정대복이마림치통소서통행위학급척수c-fos표체적영향。방법웅성성년곤명소서32지，체질량20～25 g ，수궤분위4조，매조8지。A조재소서우측좌골신경방주사생리염수；B조재소서우족저피하주사5％복이마림（10μL ），건립급성염성통모형；C조재소서치통전15 min예선재우측좌골신경방주사우미탁미정0．1μg ；D조재소서우측좌골신경방주사우미탁미정0．1μg。관찰각조소서Ⅰ、Ⅱ시상첨교족시간，재2 h시간점처사소서，취요L4～5단척수，행면역형광검측c-fos적표체。결과여A조비교，B조소서재Ⅰ、Ⅱ시상출현명현적첨교족행위，동통평분명현증고，척수c-fos적표체명현증다（P＜0．05）；여B조비교，C조첨교족시간명현축단，동통평분명현강저、척수c-fos적표체명현감소（P＜0．05）；A、C、D조동통평분화척수c-fos적표체차이무통계학의의。결론좌골신경방주사우미탁미정능구감경소서족저주사복이마림인기적급성염성통，병차강저척수c-fos적표체。
Objective To investigate the effect of sciatic nerve injection of dexmedetomidine on the nociceptive behavior and spi-nal c-fos expression in formalin mice .Methods Adult male Kunming mice ,20-25 g ,were divided into 4 groups randomly :mice in group A and D were given an injection of NS (10 μL) and 0 .1 μg dexmedetomidine in 10 μL adjacent to the right sciatic nerve , sepretely .Mice in group B received intacutaneous injection of 10μL formalin(5% ) in the right hindpaws .Mice in group C were giv-en an injection of 0 .1 μg dexmedetomidine in 10 μL adjacent to the right sciatic nerve 15 min before formalin injection in the right hindpaws .Paw licking/biting time was counted every 5 min for an hour after hindpaw injections ,calculated pain score .1 hour after behavior tests ,the L4-5 of spinal cord were harvested to immunofluorescence for c-fos expression in the dorsal corn of spinal cord . Results Mice in group A、C and D showed no obvious foot licking/biting behaviors and a low level of c-fos expression in the dorsal corn of spinal cord .Compared with group A ,mice in group B displayed obvious two phases foot licking/biting behaviors(P<0 .05 , both in Phase Ⅰ and Phase Ⅱ) and a significant increasing expression of c-fos(P<0 .05) .Compared with group B ,dexmedetomi-dine injected to the adjacent of right sciatic nerve decreased the pain score and spinal c-fos expression in goup C(P<0 .05) .Conclu-sion Injection of dexmedetomidine to the right sciatic nerve alleviated intraplantar injected formalin-induced acute inflammatory pain ,and reduced the expression of spinal c-fos protein .