CAJ | 학술논문

目的：本文旨在明确miR-216a是否通过靶向调控PKCα表达而抑制胃癌细胞增殖和促进其凋亡，从而进一步揭示miR-216a的抑瘤分子机制。方法首先构建PKCα3′U T R-荧光素酶报告载体，通过荧光素酶报告检测观察miR-216a对 PKCα3′UTR-荧光素酶活性的影响；将miR-216a模拟物转染胃癌细胞MGC-803，采用Western blot检测PKCα蛋白表达水平；将PKCαsiRNA转染MGC-803，通过MTS细胞增殖活性检测和Annexin V-FITC/PI凋亡实验考察PKCα下调对MGC-803增殖和凋亡的影响。结果荧光素酶报告检测显示，miR-216a能特异性地与 PKCα3′U T R结合，抑制其荧光素酶活性，下调36％。过表达miR-216a的MGC-803 PKCα蛋白表达水平降低。siRNA干扰 PKCα表达能抑制 MGC-803的增殖和侵袭能力，它能部分模拟miR-216a的抑瘤功能。结论 miR-216a通过靶向PKCαmRNA 3′UTR而抑制胃癌细胞增殖和侵袭能力。
목적：본문지재명학miR-216a시부통과파향조공PKCα표체이억제위암세포증식화촉진기조망，종이진일보게시miR-216a적억류분자궤제。방법수선구건PKCα3′U T R-형광소매보고재체，통과형광소매보고검측관찰miR-216a대 PKCα3′UTR-형광소매활성적영향；장miR-216a모의물전염위암세포MGC-803，채용Western blot검측PKCα단백표체수평；장PKCαsiRNA전염MGC-803，통과MTS세포증식활성검측화Annexin V-FITC/PI조망실험고찰PKCα하조대MGC-803증식화조망적영향。결과형광소매보고검측현시，miR-216a능특이성지여 PKCα3′U T R결합，억제기형광소매활성，하조36％。과표체miR-216a적MGC-803 PKCα단백표체수평강저。siRNA간우 PKCα표체능억제 MGC-803적증식화침습능력，타능부분모의miR-216a적억류공능。결론 miR-216a통과파향PKCαmRNA 3′UTR이억제위암세포증식화침습능력。
Objective To confirm whether miR-216a suppresses cell proliferation and induces cell apoptosis by targeting PKCα, thus to reveal molecular mechanism that miR-216a functions as a tumor suppressor in gastric cancer .Methods PKCα3′untranslat-ed region(UTR)-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-216a on luciferase activity .MGC-803 cells were transfected with miR-216a mimics ,and next Western blotting was performed to detect the expression of PKCαprotein .The effects of PKCαdownregulation on cell proliferation and apoptosis were observed after PKCαsiRNA were transfected into MGC-803 cells .MGC-803 cell proliferation assays were performed when cotransfected with miR-216a mimics .Results The result demonstrated miR-216a could bind to the 3′UTR of PKCαand inhibited the luciferase activi-ty ,cut the 41% .PKCαprotein expressions were significantly down-regulated when miR-216a was overexpressed in MGC-803 .siR-NA-mediated downregulation of PKCα could suppress the potentials of cell proliferation and induce apoptosis .Conclusion miR-216a suppresses cell proliferation and induces apoptosis by targeting PKCαmRNA 3′UTR in gastric cancer .