CAJ | 학술논문

目的：研究骨桥蛋白（OPN）和基质金属蛋白酶-2（MMP-2）在肝泡型棘球蚴（泡肝）组织中的表达，探讨OPN和MMP-2在泡肝侵袭转移中的作用。方法采用免疫组化SP法检测69例泡肝组织和12例正常肝组织（取自肝血管瘤手术切除的正常肝组织）中OPN与MMP-2的表达情况，并分析其表达与临床病理特征之间的关系。结果OPN和MMP-2主要分布在泡肝囊泡壁周围肉芽肿炎症细胞和周边部分基质细胞及部分肝细胞中，其阳性表达程度以泡肝病灶与正常肝组织交界的浸润缘处最明显。泡肝组织中OPN、MMP-2阳性表达率72．5％（50/69）和59．4％（41/69），明显高于正常肝组织中的16．7％（2/12）和8．3％（1/12），差异有统计学意义（P＜0．01）；在发生转移的泡肝组织中OPN和MMP-2阳性表达率分别为86．8％（33/38）和76．3％（29/38），明显高于无转移的54．8％（17/31）和38．7％（12/31），差异有统计学意义（P＜0．01）。OPN和MMP-2的阳性表达与泡肝患者的性别、年龄、民族、HBsAg状态、病灶大小等因素无关。OPN和MMP-2的表达呈显著正相关（r＝0．36，P＜0．01）。结论OPN和MMP-2的表达可能与泡肝的侵袭转移有关。
목적：연구골교단백（OPN）화기질금속단백매-2（MMP-2）재간포형극구유（포간）조직중적표체，탐토OPN화MMP-2재포간침습전이중적작용。방법채용면역조화SP법검측69례포간조직화12례정상간조직（취자간혈관류수술절제적정상간조직）중OPN여MMP-2적표체정황，병분석기표체여림상병리특정지간적관계。결과OPN화MMP-2주요분포재포간낭포벽주위육아종염증세포화주변부분기질세포급부분간세포중，기양성표체정도이포간병조여정상간조직교계적침윤연처최명현。포간조직중OPN、MMP-2양성표체솔72．5％（50/69）화59．4％（41/69），명현고우정상간조직중적16．7％（2/12）화8．3％（1/12），차이유통계학의의（P＜0．01）；재발생전이적포간조직중OPN화MMP-2양성표체솔분별위86．8％（33/38）화76．3％（29/38），명현고우무전이적54．8％（17/31）화38．7％（12/31），차이유통계학의의（P＜0．01）。OPN화MMP-2적양성표체여포간환자적성별、년령、민족、HBsAg상태、병조대소등인소무관。OPN화MMP-2적표체정현저정상관（r＝0．36，P＜0．01）。결론OPN화MMP-2적표체가능여포간적침습전이유관。
Objective To investigate the expression of osteopontin (OPN)and matrix metalloproteinase-2(MMP-2)in hepatic alveolar echinococcosis(HAE) ,and explore the role of OPN and MMP-2 in the invasion and metastasis of HAE infection .Methods Expression of OPN and MMP-2 in HAE tissues from 69 patients within12 nomral liver tissues .HAE were detected using SP im-munohistochemical technique ,the correlation between OPN and MMP-2 expression and clinieopahtologic features was analyzed .Re-sults OPN and MMP-2 mainly distributed in the cyst wall about granuloma inflammatory cells of HAE and and the peripheral por-tion of stromal cell and liver cells ,the positive expression rate in HAE focus and normal liver tissue at the juncti-on of the invasive margin the most obvious .The results showed that the positive rates of OPN and MMP-2 in HAE tissue were 72 .5% (50/69)and 59 .4% (41/69) ,respectively ,and in normal liver tissue were 16 .7% (2/12) and 8 .3% (1/12) ,respectively .The positive rates of OPN and MMP-2 in HAE were significantly higher than those in normal liver tissue(P<0 .01) .The positive rates of OPN and P21 in HAE tissues with metastasis were 86 .8% (33/38)and 76 .3% (29/38) ,respectively ,which were significantly higher than those without metastasis of OPN and MMP-2 positive expression rate of 54 .8% (17/31) and 38 .7% (12/31) ,(P<0 .01) .The positive expression of OPN and MMP-2 were not related to the size of tumor bulk ,HBsAg ,gender ,age and nation of tumor .Rank correlated analysis showed that OPN and MMP-2 were positive correlated(r=0 .36 ,P<0 .01) .Conclusion OPN and MMP-2 mainly distrib-utes in the cyst wall about granuloma inflammatory cells of HAE and and the peripheral portion of liver cells ,the positive expres-sion rate in HAE and normal liver tissue at the junction of the invasive margin the most obvious ,which might be invasion and me-tastasis of HAE .