CAJ | 학술논문

目的：探讨整合素联接激酶（IL K ）在失血性休克大鼠心肌收缩功能中的调节作用。方法采用离体乳头肌灌流和心脏灌流技术，用不同方法预处理IL K ，测定失血性休克大鼠离体左心室乳头肌等长收缩张力的变化，以及大鼠离体心脏血流动力学指标的变化。结果休克大鼠心脏血流动力学指标随着休克时间延长而逐渐下降，休克1、2h的血流动力学指标显著低于对照组（P＜0．05）；离体左心室乳头肌收缩力呈现相同的变化趋势（P＜0．05）；休克0．5、1．0、2．0hILK活性显著低于对照组（P＜0．05）。以休克1．0 h的离体乳头肌和心脏为研究对象，ILK激动剂预处理可显著改善乳头肌的收缩力和心脏血流动力学指标（P＜0．05），而ILK特异性抑制剂则明显拮抗这种改善作用（P＜0．05）。结论 ILK在休克心肌收缩功能调节中发挥了重要作用，增强IL K的活性可增强心肌收缩功能。
목적：탐토정합소련접격매（IL K ）재실혈성휴극대서심기수축공능중적조절작용。방법채용리체유두기관류화심장관류기술，용불동방법예처리IL K ，측정실혈성휴극대서리체좌심실유두기등장수축장력적변화，이급대서리체심장혈류동역학지표적변화。결과휴극대서심장혈류동역학지표수착휴극시간연장이축점하강，휴극1、2h적혈류동역학지표현저저우대조조（P＜0．05）；리체좌심실유두기수축력정현상동적변화추세（P＜0．05）；휴극0．5、1．0、2．0hILK활성현저저우대조조（P＜0．05）。이휴극1．0 h적리체유두기화심장위연구대상，ILK격동제예처리가현저개선유두기적수축력화심장혈류동역학지표（P＜0．05），이ILK특이성억제제칙명현길항저충개선작용（P＜0．05）。결론 ILK재휴극심기수축공능조절중발휘료중요작용，증강IL K적활성가증강심기수축공능。
Objective To explore the effect of ILK on myocardium contraction following hemorrhagic shock .Methods With iso-lated cardiac papillary muscle and isolated heart ,the contraction of papillary muscle and hemodynamic parameters (left intraventricu-lar systolic pressure(LVSP) ,the maximal change rate of left intraventricular pressure were measured .Results Compared with nor-mal control hearts ,ILK activity ,contractile response of cardiac papillary muscle and hemodynamic parameter were decreased signifi-cantly gradually in shock heart at the 1 ,2 h(P<0 .05) ,and the change of ILK activity was positive correlative with contractile re-sponse and hemodynamic parametert .With ILK agonist [phosphatidylinositol(3 ,4 ,5)trisphosphate ,PLTP] the dysfunction of con-tractile response and hemodynamic parameter could be improved significantly (P<0 .05) ,while these improvement could be abol-ished by ILK specific inhibitor(P<0 .05) .Conclusion ILK play important role in the regulation of cardiac contractility following hemorrhagic shock .