CAJ | 학술논문

目的：探讨在丙型肝炎患者早期诊断中丙型肝炎病毒抗体联合丙型肝炎病毒核酸（HCV RNA）检测的临床价值。方法对116例鼓楼医院住院及门诊丙型肝炎患者采用实时荧光定量聚合酶链反应（FQ-PCR）测定HCV RNA、酶联免疫吸附试验（ELISA）和金标法测 HCV抗体、速率法测丙氨酸氨基转移酶（ALT ）。结果116例丙型肝炎患者中，ELISA HCV抗体阳性97例（占83．6％），HCV RNA阳性85例（占73．3％），HCV 金标阳性77例（占66．4％），ALT阳性69例（占59．5％），HCV抗体和 HCV RNA联合检测总阳性率（指任一指标阳性即为阳性）为100．0％。结论 HCV抗体和HCV RNA联合检测扩大了丙型肝炎检测范围，降低了丙型肝炎的漏诊率，有利于丙型肝炎的早期诊断。
목적：탐토재병형간염환자조기진단중병형간염병독항체연합병형간염병독핵산（HCV RNA）검측적림상개치。방법대116례고루의원주원급문진병형간염환자채용실시형광정량취합매련반응（FQ-PCR）측정HCV RNA、매련면역흡부시험（ELISA）화금표법측 HCV항체、속솔법측병안산안기전이매（ALT ）。결과116례병형간염환자중，ELISA HCV항체양성97례（점83．6％），HCV RNA양성85례（점73．3％），HCV 금표양성77례（점66．4％），ALT양성69례（점59．5％），HCV항체화 HCV RNA연합검측총양성솔（지임일지표양성즉위양성）위100．0％。결론 HCV항체화HCV RNA연합검측확대료병형간염검측범위，강저료병형간염적루진솔，유리우병형간염적조기진단。
Objective To explore the value of hepatitis C virus antibody (HCV Ab) and hepatitis C virus nu-cleic acid (HCV RNA ) for the diagnosis of hepatitis C .Methods Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect HCV RNA ,enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeled method was used to detect HCV Ab ,and the rate method was used to detect alanine aminotransferase (ALT ) in 116 patients with hepatitis C .Results The positive ratios of HCV Ab detected by ELISA ,HCV RNA , HCV Ab detected by colloidal gold labeled method and ALT were respectively 83 .6% ,73 .3% ,66 .4% and 59 .5% . Positive rated of joint determination of HCV Ab and HCV RNA was 100 .0% .Conclusion Combined detection of HCV Ab and HCV RNA could be conducive to the diagnosis of hepatitis C .