CAJ | 학술논문

目的：探讨七氟烷对脂多糖（LPS）诱导肺部炎性损伤的保护作用及机理。方法将C57L/B6小鼠置于分别置于密闭的玻璃容器内，吸入七氟烷后腹腔注射LPS（Sevo+LPS组），6 h后处死小鼠大体观察肺部改变、测量肺重和肺湿重/干重比（W/D），HE染色观察小鼠肺部病理变化。心脏采血后检测血清中IFN-γ和IL-10水平变化。设立吸入氧后腹腔注射LPS组（O2+LPS组）和吸入氧后腹腔注射PBS组（O2+PBS组）作为对照。结果七氟烷吸入可减轻LPS诱导小鼠肺部水肿， O2+PBS组W/D值为3.96±0.23；O2+LPS组W/D值为5.73±0.43，与O2+PBS组相比明显增高（P＜0.01）；Sevo+LPS组W/D值为4.62±0.41，比O2+LPS组明显降低（P＜0.01）。HE染色肺组织切片发现七氟烷吸入可减轻LPS诱导小鼠肺组织水肿和渗出。七氟烷吸入可降低小鼠血清IFN-γ水平和提高血清IL-10的水平。结论七氟烷可能通过改变IFN-γ和IL-10等细胞因子平衡减轻LPS诱导的肺部炎性损伤。
목적：탐토칠불완대지다당（LPS）유도폐부염성손상적보호작용급궤리。방법장C57L/B6소서치우분별치우밀폐적파리용기내，흡입칠불완후복강주사LPS（Sevo+LPS조），6 h후처사소서대체관찰폐부개변、측량폐중화폐습중/간중비（W/D），HE염색관찰소서폐부병리변화。심장채혈후검측혈청중IFN-γ화IL-10수평변화。설립흡입양후복강주사LPS조（O2+LPS조）화흡입양후복강주사PBS조（O2+PBS조）작위대조。결과칠불완흡입가감경LPS유도소서폐부수종， O2+PBS조W/D치위3.96±0.23；O2+LPS조W/D치위5.73±0.43，여O2+PBS조상비명현증고（P＜0.01）；Sevo+LPS조W/D치위4.62±0.41，비O2+LPS조명현강저（P＜0.01）。HE염색폐조직절편발현칠불완흡입가감경LPS유도소서폐조직수종화삼출。칠불완흡입가강저소서혈청IFN-γ수평화제고혈청IL-10적수평。결론칠불완가능통과개변IFN-γ화IL-10등세포인자평형감경LPS유도적폐부염성손상。
Objective To explore the protective effect of sevolfurane to LPS induced acute lung injury and the underlying mechanism. Methods C57L/B6 mice were put into a sealed glass chamber administrated LPS with intraperitoneal injection after inhaled sevolfurane (Sevo+LPS group). 6h later, mice were sacriifced to observe the pathological changes, measure the weight of lung and calculate the wet weight/dry weight ratio of lung. And then, the serum levels of IFN-γand IL-10 were assayed with ELISA after hemospasia from heart. Administrated LPS with intraperitoneal injection after inhaled oxygen (O2+LPS group) and administrated PBS with intraperitoneal injection after inhaled oxygen (O2+PBS group) were set up as control groups. Results Sevolfurane could relief the LPS induced lung edema. The W/D ratio of O2+PBS group was 3.96±0.23, and the W/D ratio of O2+LPS group was 5.73±0.43. And the W/D ratio of Sevo+LPS group was 4.62±0.41, less than that of O2+LPS group (P<0.01). Lung tissue stained with HE indicated that sevolfurane could decrease the edema and exudation of lung. ELISA assay showed that sevolfurane could inhibit the serum level of IFN-γand enhanced the serum level of IL-10. Con-clusion Sevolfurane might relief the lung inlfammatory injury induced with LPS by altering the balance of cyto-kines.