中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
12期
953-955
,共3页
毒死蜱%工作场所%色谱法,高效液相
毒死蜱%工作場所%色譜法,高效液相
독사비%공작장소%색보법,고효액상
Workplace air%Chlorpyrifos%High performance liquid chromatography
目的 建立工作场所空气中毒死蜱的高效液相色谱测定方法.方法 采用硅胶管吸附空气中的毒死蜱,用乙腈解吸,高效液相色谱-紫外检测器测定.结果 该方法在0～10.0 μg/ml范围内呈线性关系,回归方程为y=5206.1x-104.7,相关系数r=0.9999.检出限为0.006μg/ml,最低检出浓度为0.001 mg/m3(以采集4.5 L空气样品计)；平均加标回收率为98.3％～102.5％；批内精密度为1.96％ ～4.39％,批间精密度为2.76％～5.87％；平均解吸效率为99.0％～103.3％,采样效率为94.0％,样品在室温下至少可保存15d.结论 该方法各项指标均符合GBZ/T 210.4-2008《工作场所空气中化学物质测定方法》的要求,适用于工作场所空气中毒死蜱的检测.
目的 建立工作場所空氣中毒死蜱的高效液相色譜測定方法.方法 採用硅膠管吸附空氣中的毒死蜱,用乙腈解吸,高效液相色譜-紫外檢測器測定.結果 該方法在0～10.0 μg/ml範圍內呈線性關繫,迴歸方程為y=5206.1x-104.7,相關繫數r=0.9999.檢齣限為0.006μg/ml,最低檢齣濃度為0.001 mg/m3(以採集4.5 L空氣樣品計)；平均加標迴收率為98.3％～102.5％；批內精密度為1.96％ ～4.39％,批間精密度為2.76％～5.87％；平均解吸效率為99.0％～103.3％,採樣效率為94.0％,樣品在室溫下至少可保存15d.結論 該方法各項指標均符閤GBZ/T 210.4-2008《工作場所空氣中化學物質測定方法》的要求,適用于工作場所空氣中毒死蜱的檢測.
목적 건립공작장소공기중독사비적고효액상색보측정방법.방법 채용규효관흡부공기중적독사비,용을정해흡,고효액상색보-자외검측기측정.결과 해방법재0～10.0 μg/ml범위내정선성관계,회귀방정위y=5206.1x-104.7,상관계수r=0.9999.검출한위0.006μg/ml,최저검출농도위0.001 mg/m3(이채집4.5 L공기양품계)；평균가표회수솔위98.3％～102.5％；비내정밀도위1.96％ ～4.39％,비간정밀도위2.76％～5.87％；평균해흡효솔위99.0％～103.3％,채양효솔위94.0％,양품재실온하지소가보존15d.결론 해방법각항지표균부합GBZ/T 210.4-2008《공작장소공기중화학물질측정방법》적요구,괄용우공작장소공기중독사비적검측.
Objective To establish the method of detecting the concentrations of chlorpyrifos in air of workplace with high performance liquid chromatographic (HPLC).Methods According to standards of methods for determining the chemical substances in workplace air,chlorpyrifos in the air was collected by silicone tube,then dissolved by acetonitrile and determined by high performance liquid chromatography with UV-detector.Results There was a linear relationship within the range of 0～10.0 μg/ml,and regression equation was y=5206.1x-104.7,correlation coefficient was 0.9999,the detection limit was 0.006 μg/ml.The lowest detected concentration was 0.001 mg/m3 (sampling volume 4.5 L).The average recoveries was 98.3％ ～102.5％.The within-run precision was 1.96％～4.39％,the between-run precision was 2.76％～5.87％.The desorption efficiencies were 99.0％～103.3％ and the sampling efficiencies were 94％.The samples in silicone tube could be stored for 15 days at room temperature.Conclusion The present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of chlorpyrifos in workplace air.
