CAJ | 학술논문

目的观察葛根素对去卵巢和非去卵巢阿尔茨海默病(AD)大鼠模型治疗效果. 方法 6月龄雌性SD大鼠100只,随机分成5组:空白正常组、非去卵巢AD组、去卵巢AD组、非去卵巢AD治疗组和去卵巢AD治疗组,每组20只.采用D-半乳糖联合亚硝酸钠造模60 d,用葛根素对治疗组进行治疗8周.实验结束,用水迷宫实验测大鼠学习记忆力,再处死取血清测雌二醇、孕酮、促卵泡激素和黄体生成素含量；大脑匀浆测胆碱酯酶(TchE)、超氧化物歧化酶(SOD)的活力、丙二醛(MDA)的浓度. 结果与空白正常组比较,2个模型组TchE活力、MDA的浓度升高(P＜0.01),SOD的活力明显降低(P＜0.01),学习记忆力明显下降.与模型组比较,2个治疗组TchE活力、MDA的浓度降低(P＜0.01),SOD的活力升高(P＜0.01),学习记忆力改善.与非去卵巢AD治疗组比较,去卵巢AD治疗组的SOD活力升高和TchE活力和MDA含量降低更明显(P＜0.05),学习记忆力改善更明显. 结论葛根素对AD大鼠模型有治疗效果,且对雌激素缺乏去卵巢AD大鼠模型治疗效果更好.
목적 관찰갈근소대거란소화비거란소아이자해묵병(AD)대서모형치료효과. 방법 6월령자성SD대서100지,수궤분성5조:공백정상조、비거란소AD조、거란소AD조、비거란소AD치료조화거란소AD치료조,매조20지.채용D-반유당연합아초산납조모60 d,용갈근소대치료조진행치료8주.실험결속,용수미궁실험측대서학습기억력,재처사취혈청측자이순、잉동、촉란포격소화황체생성소함량；대뇌균장측담감지매(TchE)、초양화물기화매(SOD)적활력、병이철(MDA)적농도. 결과 여공백정상조비교,2개모형조TchE활력、MDA적농도승고(P＜0.01),SOD적활력명현강저(P＜0.01),학습기억력명현하강.여모형조비교,2개치료조TchE활력、MDA적농도강저(P＜0.01),SOD적활력승고(P＜0.01),학습기억력개선.여비거란소AD치료조비교,거란소AD치료조적SOD활력승고화TchE활력화MDA함량강저경명현(P＜0.05),학습기억력개선경명현. 결론 갈근소대AD대서모형유치료효과,차대자격소결핍거란소AD대서모형치료효과경호.
Objective To observe the curative effect of puerarin on Alzheimer's disease (AD) in ovariectomized versus non-ovariectomized rat.Methods 100 6-month-old female SD rats were randomly divided into 5 groups:the control group,non ovariectomized AD group,ovariectomized AD group,non-ovariectomized AD treatment group and ovariectomized AD treatment group (n =20each).Rat model of AD was made by treating with combined D-galactose and sodium nitrite for 60days.Treatment group rats were treated with puerarin for 8 weeks.At the end of the experiment,rats learning and memory abilities were tested by water maze,the serum estradiol,progesterone,follicle stimulating hormone and luteinizing hormone were measured,and the activities of cholinesterase (TchE) and superoxide dismutase (SOD),malondialdehyde (MDA) concentration in brain homogenate were determined.Results Compared with the control group,TchE activity and MDA content were increased,and SOD activity was reduced (all P＜0.01),and learning and memory abilities were significantly decreased in the ovariectomized and non-ovariectomized AD groups.Compared with the model groups,the activity of TchE and MDA concentration were reduced,and SOD activity was increased (all P＜0.01),and learning and memory abilities were significantly improved in the two treatment groups.As compared with non ovariectomized AD treatment group,the increase in SOD and TchE activities and decrease in MAD concentration were much more in ovariectomized AD treatment group (P＜0.05),and the improvement in learning and memory abilities was more significant.Conclusions Puerarin has a therapeutic effect on rat model of AD,even better on the ovariectomized rat model of AD.