CAJ | 학술논문

目的观察骨桥蛋白(OPN)在豚鼠胆囊胆固醇结石模型中的动态变化.方法将44只豚鼠随机分为胆固醇致石组和对照组,于饲养后1周、2周、4周、6周、8周和10周分别处死.实时PCR测定OPN在胆囊和肝脏组织中的表达,酶联免疫吸附法(EHSA)测定OPN、黏蛋白、α1-酸性糖蛋白(AAP)和载脂蛋白A1(APOA1)在胆汁和血浆中的变化.结果 OPN mRNA在豚鼠肝脏和胆囊组织中的表达变化一致,逐渐增高至第6周达到高峰,随后又逐渐下降.致石组胆汁中OPN合量从第1周开始上升,至第6周达最大值(P＜0.05),随后OPN浓度逐渐下降,至第10周,OPN含量已降至基线水平(P＞0.05).血浆OPN浓度变化与胆汁一致,但血浆OPN高峰出现早(第4周),随即逐渐下降至基线水平(P＞0.05).豚鼠胆结石形成过程中,APOAI在胆汁和血浆中的变化与OPN类似,但其血浆峰值没有提前.黏蛋白和AAP在造模开始后即逐渐升高,从第4周开始明显增高,随后继续升高并维持在一个高水平.结论 OPN参与胆囊胆固醇结石形成的整个过程,OPN与其他成核活性因子可能具有相互作用.
목적 관찰골교단백(OPN)재돈서담낭담고순결석모형중적동태변화.방법 장44지돈서수궤분위담고순치석조화대조조,우사양후1주、2주、4주、6주、8주화10주분별처사.실시PCR측정OPN재담낭화간장조직중적표체,매련면역흡부법(EHSA)측정OPN、점단백、α1-산성당단백(AAP)화재지단백A1(APOA1)재담즙화혈장중적변화.결과 OPN mRNA재돈서간장화담낭조직중적표체변화일치,축점증고지제6주체도고봉,수후우축점하강.치석조담즙중OPN합량종제1주개시상승,지제6주체최대치(P＜0.05),수후OPN농도축점하강,지제10주,OPN함량이강지기선수평(P＞0.05).혈장OPN농도변화여담즙일치,단혈장OPN고봉출현조(제4주),수즉축점하강지기선수평(P＞0.05).돈서담결석형성과정중,APOAI재담즙화혈장중적변화여OPN유사,단기혈장봉치몰유제전.점단백화AAP재조모개시후즉축점승고,종제4주개시명현증고,수후계속승고병유지재일개고수평.결론 OPN삼여담낭담고순결석형성적정개과정,OPN여기타성핵활성인자가능구유상호작용.
Objective To investigate the dynamic changes of osteopontin (OPN) in the guinea pig model of cholesterol gallstone disease.Methods Forty-four guinea pigs were randomly assigned to cholesterol gallstone group and control group.The animals were sequentially killed on Day 7,14,28,42,56 and 70 after operation.The expressions of OPN mRNA in gallbladder and liver tissues were detected by real-time PCR.The changes of OPN,mucin,α1-acid glycoprotein (AAP) and apolipoprotein A1 (APOA1) in bile and blood plasma were detected by ELISA kits.Results The expression of OPN mRNA in gallbladder and liver tissues increased gradually and reached the peak in the 6th week,and then decreased.The increased expression of OPN in bile in the gallstone group started from the 1st week and reached the peak in the 6th week (P ＜ 0.05),which gradually decreased to the baseline in the 10th week (P ＞ 0.05).The expressions of OPN in bile and blood demonstrated similar trends,while the peak time in blood samples was much earlier (4th week).The changes of APOA1 in bile and blood were similar to OPN,although there was no advanced peak value in blood.The levels of mucin and AAP in bile and blood increased after operation,and were kept at high level throughout the study.Conclusions OPN is involved in the whole process of cholesterol gallstone formation,which may be associated with other nucleation-active factors.