中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
12期
859-861
,共3页
谢欣%盖希余%秦晓娟%张琳%柴立
謝訢%蓋希餘%秦曉娟%張琳%柴立
사흔%개희여%진효연%장림%시립
扁平苔藓%细胞凋亡%半胱氨酸天冬氨酸蛋白酶3%卡配因
扁平苔蘚%細胞凋亡%半胱氨痠天鼕氨痠蛋白酶3%卡配因
편평태선%세포조망%반광안산천동안산단백매3%잡배인
Lichen planus%Apoptosis%Caspase 3%Calpain
目的 探讨凋亡相关蛋白钙激活蛋白酶Ⅰ (Calpain Ⅰ)和半胱氨酸天冬氨酸蛋白酶3(Caspase-3)在扁平苔藓皮损中的表达及其与细胞凋亡的关系.方法 分别采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记(TUNEL)法和免疫组化法对20例扁平苔藓患者皮损组织和10例健康人皮肤组织中细胞凋亡和Calpain Ⅰ、Caspase-3表达进行检测.采用SPSS 13.0软件,两组凋亡指数比较采用t检验,两组Calpain Ⅰ和Caspase-3表达量比较采用秩和检验,Calpain Ⅰ和Caspase-3表达量与凋亡指数相关性分析采用Spearman等级相关分析.结果 扁平苔藓组表皮层角质形成细胞的凋亡指数(67.59±13.50)显著高于健康对照组(28.26±7.56),两组比较,差异有统计学意义(t=8.52,P＜0.01)；Calpain Ⅰ和Caspase-3表达均高于健康对照组(T=78.00和77.00,P＜0.01).扁平苔藓皮损中Calpain Ⅰ和Caspase-3阳性表达强度均与其自身角质形成细胞的凋亡指数呈正相关(r=0.71和0.74,P＜ 0.01).结论 扁平苔藓皮损中Calpain Ⅰ和Caspase-3表达上调,且与角质形成细胞凋亡亢进关系密切.
目的 探討凋亡相關蛋白鈣激活蛋白酶Ⅰ (Calpain Ⅰ)和半胱氨痠天鼕氨痠蛋白酶3(Caspase-3)在扁平苔蘚皮損中的錶達及其與細胞凋亡的關繫.方法 分彆採用脫氧覈糖覈苷痠末耑轉移酶介導的原位缺口末耑標記(TUNEL)法和免疫組化法對20例扁平苔蘚患者皮損組織和10例健康人皮膚組織中細胞凋亡和Calpain Ⅰ、Caspase-3錶達進行檢測.採用SPSS 13.0軟件,兩組凋亡指數比較採用t檢驗,兩組Calpain Ⅰ和Caspase-3錶達量比較採用秩和檢驗,Calpain Ⅰ和Caspase-3錶達量與凋亡指數相關性分析採用Spearman等級相關分析.結果 扁平苔蘚組錶皮層角質形成細胞的凋亡指數(67.59±13.50)顯著高于健康對照組(28.26±7.56),兩組比較,差異有統計學意義(t=8.52,P＜0.01)；Calpain Ⅰ和Caspase-3錶達均高于健康對照組(T=78.00和77.00,P＜0.01).扁平苔蘚皮損中Calpain Ⅰ和Caspase-3暘性錶達彊度均與其自身角質形成細胞的凋亡指數呈正相關(r=0.71和0.74,P＜ 0.01).結論 扁平苔蘚皮損中Calpain Ⅰ和Caspase-3錶達上調,且與角質形成細胞凋亡亢進關繫密切.
목적 탐토조망상관단백개격활단백매Ⅰ (Calpain Ⅰ)화반광안산천동안산단백매3(Caspase-3)재편평태선피손중적표체급기여세포조망적관계.방법 분별채용탈양핵당핵감산말단전이매개도적원위결구말단표기(TUNEL)법화면역조화법대20례편평태선환자피손조직화10례건강인피부조직중세포조망화Calpain Ⅰ、Caspase-3표체진행검측.채용SPSS 13.0연건,량조조망지수비교채용t검험,량조Calpain Ⅰ화Caspase-3표체량비교채용질화검험,Calpain Ⅰ화Caspase-3표체량여조망지수상관성분석채용Spearman등급상관분석.결과 편평태선조표피층각질형성세포적조망지수(67.59±13.50)현저고우건강대조조(28.26±7.56),량조비교,차이유통계학의의(t=8.52,P＜0.01)；Calpain Ⅰ화Caspase-3표체균고우건강대조조(T=78.00화77.00,P＜0.01).편평태선피손중Calpain Ⅰ화Caspase-3양성표체강도균여기자신각질형성세포적조망지수정정상관(r=0.71화0.74,P＜ 0.01).결론 편평태선피손중Calpain Ⅰ화Caspase-3표체상조,차여각질형성세포조망항진관계밀절.
Objective To quantify the expression levels of calpain 1 and caspase-3 in lichen planus (LP) lesions and their relationship with the apoptosis in keratinocytes.Methods Biopsy samples were obtained from the lesions of 20 patients with LP and normal skin of 10 healthy controls,and embedded in paraffin.Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was used to evaluate the apoptosis in keratinocytes,and immunohistochemical staining to detect the expressions of calpain 1 and caspase-3,in these tissue specimens.Data were processed by SPSS 13.0 software.Comparison analysis was carried out by t test for apoptosis index,and by rank sum test for the expressions of calpain 1 and caspase-3.Spearman correlation analysis was conducted to evaluate the relationship between these parameters.Results The apoptosis index of keratinocytes was higher in LP lesions than in the normal skin (67.59 ± 13.50 vs.28.26 ± 7.56,t =8.52,P ＜ 0.01).Significantly increased expressions of calpain 1 and caspase-3 were observed in the epidermis of LP lesions compared with the normal skin (T =78.00 and 77.00,respectively,both P ＜ 0.01).The expressions of both calpain 1 and caspase-3 were positively correlated with apoptosis index of keratinocytes (r =0.71 and 0.74,respectively,both P ＜ 0.01).Conclusions The expressions of calpain 1 and caspase-3 are upregulated in LP lesions,which may be closely associated with the accelerated apoptosis in keratinocytes.
