CAJ | 학술논문

为了明确球孢白僵菌对玉米象的毒力,为玉米象生物防治提供依据,本研究采用浸渍法生物测定了球孢白僵菌Bb10716菌株在30,27,24,21,18,15℃下对玉米象Sitophilus zeamais(Coleoptera:Curculionidae)成虫的毒力.结果表明,在30,27,24,21,18,15℃条件下,接种Bb10716菌株1.76×108 ～ 1.76×103孢子/mL孢子悬浮液后第12天时玉米象成虫的校正死亡率分别为(2.22±0.21)％～(61.11±2.47)％,(3.33±0.26)％～(96.67 ±2.45)％,(6.67 ±0.23)％～(98.76 ±0.45)％,(6.67 ±0.23)％～ (98.86 ±0.65)％,(0～56.67±1.54)％,(0～46.67 ±2.45)％.在30,27,24,21,18,15℃下,菌株Bb10716对玉米象成虫的毒力回归方程分别是Y=1.716 +0.442X (R2 =0.93),Y=0.81+0.74X(R2 =0.92),Y=0.73+0.84X(R2=0.87),Y=0.48+1.26X (R2=0.84),Y=1.15+0.38X(R2=0.86)和Y=1.32 +0.50X (R2 =0.94),对玉米象成虫的致死中浓度LC50分别是2.68×107,4.49×105,1.20×105,1.70×105,9.16×107,8.33×10 7孢子/mL.用1.72×108孢子/mL孢子悬浮液接种处理后,对玉米象成虫致死中时LT50分别为(8.1±0.25),(6.5 ±0.36),(7.5±0.54),(7.1 ±0.54),(7.8±0.48),(11.6±1.12)d.由此表明,球孢白僵菌Bb10716菌株对玉米象成虫具有较强的毒力,在21,24,27℃下毒力最高,Bb10716菌株可作为玉米象成虫生物制剂开发用菌株.
위료명학구포백강균대옥미상적독력,위옥미상생물방치제공의거,본연구채용침지법생물측정료구포백강균Bb10716균주재30,27,24,21,18,15℃하대옥미상Sitophilus zeamais(Coleoptera:Curculionidae)성충적독력.결과표명,재30,27,24,21,18,15℃조건하,접충Bb10716균주1.76×108 ～ 1.76×103포자/mL포자현부액후제12천시옥미상성충적교정사망솔분별위(2.22±0.21)％～(61.11±2.47)％,(3.33±0.26)％～(96.67 ±2.45)％,(6.67 ±0.23)％～(98.76 ±0.45)％,(6.67 ±0.23)％～ (98.86 ±0.65)％,(0～56.67±1.54)％,(0～46.67 ±2.45)％.재30,27,24,21,18,15℃하,균주Bb10716대옥미상성충적독력회귀방정분별시Y=1.716 +0.442X (R2 =0.93),Y=0.81+0.74X(R2 =0.92),Y=0.73+0.84X(R2=0.87),Y=0.48+1.26X (R2=0.84),Y=1.15+0.38X(R2=0.86)화Y=1.32 +0.50X (R2 =0.94),대옥미상성충적치사중농도LC50분별시2.68×107,4.49×105,1.20×105,1.70×105,9.16×107,8.33×10 7포자/mL.용1.72×108포자/mL포자현부액접충처리후,대옥미상성충치사중시LT50분별위(8.1±0.25),(6.5 ±0.36),(7.5±0.54),(7.1 ±0.54),(7.8±0.48),(11.6±1.12)d.유차표명,구포백강균Bb10716균주대옥미상성충구유교강적독력,재21,24,27℃하독력최고,Bb10716균주가작위옥미상성충생물제제개발용균주.