国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2012年
6期
361-364
,共4页
微RNAs%肝癌%肿瘤标志物
微RNAs%肝癌%腫瘤標誌物
미RNAs%간암%종류표지물
MicroRNAs%Liver tumor%Tumor marker
目的 探讨血浆中miR-199a/b-3p表达水平是否可以应用于肝癌的筛查.方法 采用realtime PCR方法及统计学方法分析肝癌患者(90例)和健康人(50例)的miR-199a/b-3p水平,分析肝癌手术前后(44例)miR-199a/b-3p表达水平,肝癌不同分期血浆中miR-199a/b-3p表达水平的差异,以及miR-199a/b-3p表达水平与肝癌患者存活期的相关性.结果 肝癌患者血浆中miR-199a/b-3p表达水平明显低于健康人(t=-4.557,P＜0.01)；经手术切除肿瘤之后,患者血浆中miR-199a/b-3p表达水平较术前明显上升(t=-0.414,P＜ 0.01)；不同分期肝癌患者血浆中miR-199a/b-3p表达水平差异无统计学意义(F=0.258,P＞0.05)；术后miR-199a/b-3p表达水平与患者的存活时间正相关(r=0.788,P＜0.01).结论 通过检测血浆中miR-199a/b-3p表达水平可以对肝癌患者进行筛查,并对术后患者的愈后情况进行判断.
目的 探討血漿中miR-199a/b-3p錶達水平是否可以應用于肝癌的篩查.方法 採用realtime PCR方法及統計學方法分析肝癌患者(90例)和健康人(50例)的miR-199a/b-3p水平,分析肝癌手術前後(44例)miR-199a/b-3p錶達水平,肝癌不同分期血漿中miR-199a/b-3p錶達水平的差異,以及miR-199a/b-3p錶達水平與肝癌患者存活期的相關性.結果 肝癌患者血漿中miR-199a/b-3p錶達水平明顯低于健康人(t=-4.557,P＜0.01)；經手術切除腫瘤之後,患者血漿中miR-199a/b-3p錶達水平較術前明顯上升(t=-0.414,P＜ 0.01)；不同分期肝癌患者血漿中miR-199a/b-3p錶達水平差異無統計學意義(F=0.258,P＞0.05)；術後miR-199a/b-3p錶達水平與患者的存活時間正相關(r=0.788,P＜0.01).結論 通過檢測血漿中miR-199a/b-3p錶達水平可以對肝癌患者進行篩查,併對術後患者的愈後情況進行判斷.
목적 탐토혈장중miR-199a/b-3p표체수평시부가이응용우간암적사사.방법 채용realtime PCR방법급통계학방법분석간암환자(90례)화건강인(50례)적miR-199a/b-3p수평,분석간암수술전후(44례)miR-199a/b-3p표체수평,간암불동분기혈장중miR-199a/b-3p표체수평적차이,이급miR-199a/b-3p표체수평여간암환자존활기적상관성.결과 간암환자혈장중miR-199a/b-3p표체수평명현저우건강인(t=-4.557,P＜0.01)；경수술절제종류지후,환자혈장중miR-199a/b-3p표체수평교술전명현상승(t=-0.414,P＜ 0.01)；불동분기간암환자혈장중miR-199a/b-3p표체수평차이무통계학의의(F=0.258,P＞0.05)；술후miR-199a/b-3p표체수평여환자적존활시간정상관(r=0.788,P＜0.01).결론 통과검측혈장중miR-199a/b-3p표체수평가이대간암환자진행사사,병대술후환자적유후정황진행판단.
Objective To evaluate the potential value of plasma level of miR-199a/b-3p in screening for liver tumor.Methods The differences of plasma level of miR-199a/b-3p between liver tumor patients(90 cases) and healthy controls (50 cases),before and after surgeries(44 cases),among different stages of liver tumor patients,were detected through real-time PCR statistical methods.The correlation of miR-199a/b-3p level and survival time of patients was also analyzed.Results The plasma level of miR-199a/b-3p was lower in liver tumor patients than in healthy controls (t =-4.557,P ＜ 0.01).The plasma level of miR-199a/b-3p in patients after surgery to remove the tumor was significantly higher than before surgery (t =-0.414,P＜0.01).The plasma levels of miR-199a/b-3p in 4 clinical stages were no statistical significances(F=0.258,P ＞ 0.05),and in postoperative patients and the survival time had a significantly positive correlation (r =0.788,P ＜ 0.01) after operation.Conclusions The plasma level of miR-199a/b-3p can be used in screening liver tumor and predicting prognosis.
