CAJ | 학술논문

目的：探讨全自动毛细管电泳技术在地中海贫血筛查中的临床诊断价值。方法选取2008年9月-2012年4月我院筛查的380例患者作为研究对象，分别采用琼脂糖凝胶电泳技术和全自动毛细管电泳技术对地中海贫血进行筛查，以通过 PCR 基因诊断技术进行确诊的结果为参照，比较两种筛查技术的检出率和血常规检查结果，以明确其临床价值。结果采用 PCR 基因诊断技术在380例患者中共有108例确诊为地中海贫血。对于α-地中海贫血，全自动毛细管电泳技术的检出率为90．91％，明显高于凝胶电泳技术的检出率65．91％；对于β-地中海贫血，毛细管电泳技术和凝胶电泳技术的检出率分别为94．23％和71．15％，全自动毛细管电泳技术检出率明显高于凝胶电泳技术；地中海贫血的 MCV、CH 均值相对于异常血红蛋白病 HbE、HbCS 值稍低，差异有统计学意义（P ＜0．05）。结论全自动毛细管电泳技术在地中海贫血筛查中检出率高，具有显著的优越性，能够为我国地中海贫血诊断工作提供更有力的技术保障。
목적：탐토전자동모세관전영기술재지중해빈혈사사중적림상진단개치。방법선취2008년9월-2012년4월아원사사적380례환자작위연구대상，분별채용경지당응효전영기술화전자동모세관전영기술대지중해빈혈진행사사，이통과 PCR 기인진단기술진행학진적결과위삼조，비교량충사사기술적검출솔화혈상규검사결과，이명학기림상개치。결과채용 PCR 기인진단기술재380례환자중공유108례학진위지중해빈혈。대우α-지중해빈혈，전자동모세관전영기술적검출솔위90．91％，명현고우응효전영기술적검출솔65．91％；대우β-지중해빈혈，모세관전영기술화응효전영기술적검출솔분별위94．23％화71．15％，전자동모세관전영기술검출솔명현고우응효전영기술；지중해빈혈적 MCV、CH 균치상대우이상혈홍단백병 HbE、HbCS 치초저，차이유통계학의의（P ＜0．05）。결론전자동모세관전영기술재지중해빈혈사사중검출솔고，구유현저적우월성，능구위아국지중해빈혈진단공작제공경유력적기술보장。
Objective To investigate clinical value of automated capillary electrophoresis in screening for thalasse-mia.Methods 380 patients of obstetrics screening from September 2008 to April 2012 in our hospital were selected as research subjects with respectively agarose gel electrophoresis and automated capillary electrophoresis technique thalas-semia screening investigation.Under conducting genetic diagnosis by PCR confirmed the results as a reference,the two detection rate of screening techniques were comprised to identify their application value.Results By using PCR gene di-agnostic techniques for diagnosis and found a total of 108 cases of 380 women diagnosed with thalassemia.for the α-thalassemia,automatic capillary electrophoresis detection rate of 90.91%,was significantly higher than the detection rate of gel electrophoresis 65.91%;For β-thalassemia,capillary electrophoresis and gel electrophoresis detection rates were 94.23%,71.15%,and were significantly higher than the automated capillary electrophoresis gel electrophoresis;Mediterranean anemia MCV and MCH mean relative to the abnormal hemoglobin disease HbE,HbCS value were low-er.Difference was statistically significant (P <0.05).Conclusion Automatic capillary electrophoresis in screening for thalassemia detection rate,with significant superiority to provide more effective technical support to screen for thalasse-mia.