CAJ | 학술논문

目的：探讨尖锐湿疣(CA)患者人乳头瘤病毒(HPV)-DNA定量及分型。方法采用荧光定量聚合酶链反应(FQ-PCR)对278例CA患者疣体组织进行HPV分型和HPV-DNA定量检测。结果278例CA患者经二氧化碳激光治疗后173例治愈,105例复发。HPV亚型分析显示，278例患者的标本中均检出HPV-DNA(100.00%)，244例(87.77%)基因型为HPV6/11,7例(2.52%)为HPV16/18，27例(9.71%)为HPV6/11+16/18。对278例HPV-DNA阳性患者治疗前病毒载量进行分组比较，病毒载量为103～105拷贝/ml的患者106例，有23例患者复发；病毒载量105～107拷贝/ml的患者82例，有29例患者复发；病毒载量＞107拷贝/ml的患者90例，有53例患者复发。HPV亚型为HPV6/11的有79例复发，HPV16/18的有5例复发，HPV6/11并发HPV16/18的有21例复发。结论检测CA患者皮损HPV-DNA能区分高危型和低危型HPV感染，可对CA复发进行预测。
목적：탐토첨예습우(CA)환자인유두류병독(HPV)-DNA정량급분형。방법채용형광정량취합매련반응(FQ-PCR)대278례CA환자우체조직진행HPV분형화HPV-DNA정량검측。결과278례CA환자경이양화탄격광치료후173례치유,105례복발。HPV아형분석현시，278례환자적표본중균검출HPV-DNA(100.00%)，244례(87.77%)기인형위HPV6/11,7례(2.52%)위HPV16/18，27례(9.71%)위HPV6/11+16/18。대278례HPV-DNA양성환자치료전병독재량진행분조비교，병독재량위103～105고패/ml적환자106례，유23례환자복발；병독재량105～107고패/ml적환자82례，유29례환자복발；병독재량＞107고패/ml적환자90례，유53례환자복발。HPV아형위HPV6/11적유79례복발，HPV16/18적유5례복발，HPV6/11병발HPV16/18적유21례복발。결론검측CA환자피손HPV-DNA능구분고위형화저위형HPV감염，가대CA복발진행예측。
Objective To use lfuorescent quantitative polymerase chain reaction (FQ-PCR)for detection of human papillomavirus(HPV) DNA and HPV typing in patients with condyloma acuminatum (CA). Methods FQ-PCR for HPV typing and detection of HPV-DNA in wart tissue were performed in 278 patients with CA. Results In the 278 cases, 173 cases were cured after carbon dioxide laser treatment, but 105 cases have a relapse. HPV subtype analysis was performed. The samples of 278 cases detected were all HPV-DNA positive (100.00%), including 244 cases(87.77%) with genotype HPV6/11, 7 cases(2.52%)with genotype HPV16/18 and 27 cases(9.71%)with genotype HPV6/11and HPV 16/18. 278 HPV-DNA positive patients without being treated in groups were compared according to the HPV-DNA virus load. There were 23 recurrence cases in 106 cases with virus load 103~105 copies/ml.There were 29 recurrence cases in 82 cases with virus load 105~107 copies/ml. There were 53 recurrence cases in 90 cases with virus load>107 copies/ml. 79 recurrence cases with HPV6/11, 5 recurrence cases with HPV16/18 and 21 recurrence cases with HPV6/11 and HPV16/18 were observed. Conclusion Detecting HPV-DNA can distinguish the high-risk HPV from low-risk HPV infection, and it can predict the possibility of condyloma recurrence.