临床普外科电子杂志
臨床普外科電子雜誌
림상보외과전자잡지
Journal of General Surgery for Clinicians (Electronic Version)
2013年
2期
19-21,25
,共4页
邢艳%宋敏%史光军%李哲夫
邢豔%宋敏%史光軍%李哲伕
형염%송민%사광군%리철부
胆囊结石%黏蛋白基因(Muc-5ac)%多重耐药因子3(MDR3)
膽囊結石%黏蛋白基因(Muc-5ac)%多重耐藥因子3(MDR3)
담낭결석%점단백기인(Muc-5ac)%다중내약인자3(MDR3)
Cholesterol calculus formation%Mucin gene%MDR3
目的：探讨黏蛋白Muc-5ac基因和多重耐药因子3(MDR3)在胆囊结石形成中的作用及其相互关系。方法应用免疫组织化学法和逆转录聚合酶链反应(RT-PCR)技术检测Muc-5ac和MDR3在36例胆囊结石、20例胆囊炎和12例正常胆囊的胆囊黏膜和肝组织中的表达。结果 Muc-5ac黏蛋白在胆囊结石和胆囊炎的胆囊黏膜中表达的阳性率分别为66.7%（24/36）和40%(8/20)，显著高于正常对照组33.3%(4/12)(P<0.01)，Muc-5ac 黏蛋白表达的阳性率在胆囊结石组又显著高于胆囊炎组(66.7%vs 40%)(P<0.05)。胆囊结石的肝组织中MDR3 mRNA的表达量(1.14±0.31)极显著低于胆囊炎的肝组织(1.89±0.83)和正常肝组织(1.97±0.71)(P<0.01)，但MDR3 mRNA的表达量在胆囊炎的肝组织中与正常肝组织之间无显著性差异(1.89±0.83 vs 1.97±0.71)(P>0.05)。胆囊结石的胆囊黏膜中， Muc-5ac蛋白阳性表达者，其肝组织中MDR3 mRNA 表达量明显减少，与Muc-5ac蛋白阴性表达组相比，MDR3 mRNA的表达量在两组间差异极显著(0.82±0.17 vs 1.45±0.24)(P<0.01)。结论 Muc-5ac基因参与了胆囊结石形成的过程。在胆囊结石形成的后期阶段，MDR3与Muc-5ac基因相互作用，共同影响胆囊结石的形成。
目的：探討黏蛋白Muc-5ac基因和多重耐藥因子3(MDR3)在膽囊結石形成中的作用及其相互關繫。方法應用免疫組織化學法和逆轉錄聚閤酶鏈反應(RT-PCR)技術檢測Muc-5ac和MDR3在36例膽囊結石、20例膽囊炎和12例正常膽囊的膽囊黏膜和肝組織中的錶達。結果 Muc-5ac黏蛋白在膽囊結石和膽囊炎的膽囊黏膜中錶達的暘性率分彆為66.7%（24/36）和40%(8/20)，顯著高于正常對照組33.3%(4/12)(P<0.01)，Muc-5ac 黏蛋白錶達的暘性率在膽囊結石組又顯著高于膽囊炎組(66.7%vs 40%)(P<0.05)。膽囊結石的肝組織中MDR3 mRNA的錶達量(1.14±0.31)極顯著低于膽囊炎的肝組織(1.89±0.83)和正常肝組織(1.97±0.71)(P<0.01)，但MDR3 mRNA的錶達量在膽囊炎的肝組織中與正常肝組織之間無顯著性差異(1.89±0.83 vs 1.97±0.71)(P>0.05)。膽囊結石的膽囊黏膜中， Muc-5ac蛋白暘性錶達者，其肝組織中MDR3 mRNA 錶達量明顯減少，與Muc-5ac蛋白陰性錶達組相比，MDR3 mRNA的錶達量在兩組間差異極顯著(0.82±0.17 vs 1.45±0.24)(P<0.01)。結論 Muc-5ac基因參與瞭膽囊結石形成的過程。在膽囊結石形成的後期階段，MDR3與Muc-5ac基因相互作用，共同影響膽囊結石的形成。
목적：탐토점단백Muc-5ac기인화다중내약인자3(MDR3)재담낭결석형성중적작용급기상호관계。방법응용면역조직화학법화역전록취합매련반응(RT-PCR)기술검측Muc-5ac화MDR3재36례담낭결석、20례담낭염화12례정상담낭적담낭점막화간조직중적표체。결과 Muc-5ac점단백재담낭결석화담낭염적담낭점막중표체적양성솔분별위66.7%（24/36）화40%(8/20)，현저고우정상대조조33.3%(4/12)(P<0.01)，Muc-5ac 점단백표체적양성솔재담낭결석조우현저고우담낭염조(66.7%vs 40%)(P<0.05)。담낭결석적간조직중MDR3 mRNA적표체량(1.14±0.31)겁현저저우담낭염적간조직(1.89±0.83)화정상간조직(1.97±0.71)(P<0.01)，단MDR3 mRNA적표체량재담낭염적간조직중여정상간조직지간무현저성차이(1.89±0.83 vs 1.97±0.71)(P>0.05)。담낭결석적담낭점막중， Muc-5ac단백양성표체자，기간조직중MDR3 mRNA 표체량명현감소，여Muc-5ac단백음성표체조상비，MDR3 mRNA적표체량재량조간차이겁현저(0.82±0.17 vs 1.45±0.24)(P<0.01)。결론 Muc-5ac기인삼여료담낭결석형성적과정。재담낭결석형성적후기계단，MDR3여Muc-5ac기인상호작용，공동영향담낭결석적형성。
Objective To evaluate the function and relationship between mucin gene (Muc-5ac) and multidrug resistance 3 (MDR3) in cholesterol calculus formation. Methods The expressions of Muc-5ac and MDR3 in mucosa and liver tissue of 36 patients with cholelithiasis, 20 patients with cholecystitis and 12 patients with normal gallbladder were measured respectively with immunohistochemical stains and reverse transcriptionpolymerase chain reaction (RT-PCR). Results The positive rates of Muc-5ac in mucosa were siginificantly higher than those of in mucosa of patients with cholelithiasis(66.7%) and cholecystitis(40%) than those of in mucosa of patients with normal gallbladder(33.3%)(P<0.01), and also higher than those of in mucosa of patients with cholelithiasis 66.7%) than those of in mucosa of patients with cholecystitis(40%)(P<0.05). The mRNA expressions of MDR3 in liver were siginificantly lower in patients with cholelithiasis(1.14±0.31) than those of in liver of patients with cholecystitis (1.89±0.83) and with normal gallbladder(1.97±0.71) respectively. However, there were no difference between the cholecystitis group and the control group (1.89±0.83 vs 1.97±0.71 ) (P>0.05). The MDR3 mRNA of liver in Muc-5ac positive in mucosa with cholelithiasis were siginificantly lower than those of in Muc-5ac negative group(0.82±0.17 vs 1.45±0.24)(P<0.01). Conclusion Muc-5ac may be associated with the whole process of cholesterol calculus formation. MDR3 and Muc-1 interacted to influence the formation of cholelithiasis in the later period.
