CAJ | 학술논문

目的：探讨北京地区女性宫颈上皮细胞感染23种人乳头瘤病毒（human papillomavirus， HPV）基因型的分布情况及其对宫颈癌预防的临床意义。方法应用反向斑点杂交-基因芯片方法对1478例不同年龄段体检女性宫颈HPV感染情况进行筛查。结果1478例女性宫颈上皮细胞标本中HPV总感染率为26.05%，单一型别阳性检出率为19.4%，HPV16型是最主要的感染型别，其次是HPV58型。单一感染HPV阳性率为19.4%，二重感染阳性率为5.1%，多重感染阳性率为1.6%；高危型HPV感染阳性率为17.9%，低危型感染阳性率为4.6%，混合型感染阳性率为3.5%；≤20岁、21～30岁、31～40岁、41～50岁和＞50岁5个年龄组中HPV感染率分别为l.2%、24.0%、28.8%、30.0%和16.0%，其后4个年龄段女性在HPV单一，二重及多重感染率差异具有统计学意义（χ2=26.475，P＜0.01）；高危型与低危型感染率差异具有统计学意义（χ2=25.887，P＜0.01）。结论 HPV16、58型是北京地区30～50岁女性单一感染的主要基因型别。监测女性HPV分型对女性宫颈癌的预防及病因学研究具有重要作用。
목적：탐토북경지구녀성궁경상피세포감염23충인유두류병독（human papillomavirus， HPV）기인형적분포정황급기대궁경암예방적림상의의。방법응용반향반점잡교-기인심편방법대1478례불동년령단체검녀성궁경HPV감염정황진행사사。결과1478례녀성궁경상피세포표본중HPV총감염솔위26.05%，단일형별양성검출솔위19.4%，HPV16형시최주요적감염형별，기차시HPV58형。단일감염HPV양성솔위19.4%，이중감염양성솔위5.1%，다중감염양성솔위1.6%；고위형HPV감염양성솔위17.9%，저위형감염양성솔위4.6%，혼합형감염양성솔위3.5%；≤20세、21～30세、31～40세、41～50세화＞50세5개년령조중HPV감염솔분별위l.2%、24.0%、28.8%、30.0%화16.0%，기후4개년령단녀성재HPV단일，이중급다중감염솔차이구유통계학의의（χ2=26.475，P＜0.01）；고위형여저위형감염솔차이구유통계학의의（χ2=25.887，P＜0.01）。결론 HPV16、58형시북경지구30～50세녀성단일감염적주요기인형별。감측녀성HPV분형대녀성궁경암적예방급병인학연구구유중요작용。
Objective To detect and type human papillomavirus(HPV) by the method of dot blot hybridization reverse-gene chip for investigating their infections in women and their roles in the prevention and treatment in cervical cancer. Methods 1 478 patients from different age were enrolled and the HPV were detected and typed by dot blot hybridization reverse-gene chip. The infection rate of low-risk and high-risk types were compared and the relationship with age were analyzed. Results The total HPV infection rate was 26.05%in 1 478 cervical cell samples, the HPV infection rate of a single type was 19.4%. The predominant type from single infection with HPV was type 16(8.80%) secondly type 58(4.4%). The HPV infection rate of single type, double type and multiple type were 19.4%, 5.1%and 1.6%respectively;the HPV infection rate of high-risk type, low-risk type and mixed type were 17.9%, 4.6%and 3.5%respectively. The positive rate of HPV were 1.2%, 24.0%, 28.8%, 30.0%and 16.0%along with the age group of ≤20, 21 ~ 30, 31, 40, 41 ~ 50 and>50 years respectively, subsequently four age women in the HPV single, double and multiple infection rate difference were statistically signiifcant (χ2=26.475, P<0.01). High-risk and low-risk type infection rate difference were statistically signiifcant (χ2=25.887, P<0.01). Conclusion HPV 16, 58 are the main genotypes of 1 478 women in Beijing district. Gene chip technology had high sensitivity and speciifcity in detecting multiple HPV genotypes in cervical epithelium, which is useful for the prevention and etiology study of cervical cancer.