CAJ | 학술논문

目的探讨正常成人胰岛移植治疗糖尿病的作用。方法2012年1月至2013年1月健康成人胰腺供体共13例。获取胰腺后经胶原酶消化并进行胰岛提取纯化，将纯化后的胰岛移植到糖尿病裸鼠肾包膜下为实验组，生理盐水代替胰岛移植为对照组，各13只。术后通过检测糖尿病裸鼠的血糖变化和组织学检查来判定胰岛移植后的存活情况和纠正血糖的效果。结果成人胰腺消化后胰岛产量为纯化前（3608±403）IEQ/g，纯化后（2820±318）IEQ/g，纯度为85%。实验组84.6%（11/13）糖尿病裸鼠在移植术后第1天，血糖降至正常，对照组血糖无明显变化。两组糖尿病裸鼠存活时间：实验组存活时间35~57 d，中位存活时间46 d。对照组存活时间5~12 d，中位存活时间7 d。两组比较差异具有统计学意义,Log-rank (Mantel-Cox)值为8.74，P 值为0.0012。结论成人胰腺消化后，可得到高纯度活力正常的胰岛，并能纠正糖尿病裸鼠的高血糖。正常成人胰岛移植可能成为临床糖尿病患者的一种有效治疗方法。
목적탐토정상성인이도이식치료당뇨병적작용。방법2012년1월지2013년1월건강성인이선공체공13례。획취이선후경효원매소화병진행이도제취순화，장순화후적이도이식도당뇨병라서신포막하위실험조，생리염수대체이도이식위대조조，각13지。술후통과검측당뇨병라서적혈당변화화조직학검사래판정이도이식후적존활정황화규정혈당적효과。결과성인이선소화후이도산량위순화전（3608±403）IEQ/g，순화후（2820±318）IEQ/g，순도위85%。실험조84.6%（11/13）당뇨병라서재이식술후제1천，혈당강지정상，대조조혈당무명현변화。량조당뇨병라서존활시간：실험조존활시간35~57 d，중위존활시간46 d。대조조존활시간5~12 d，중위존활시간7 d。량조비교차이구유통계학의의,Log-rank (Mantel-Cox)치위8.74，P 치위0.0012。결론성인이선소화후，가득도고순도활력정상적이도，병능규정당뇨병라서적고혈당。정상성인이도이식가능성위림상당뇨병환자적일충유효치료방법。
Objective To investigate the efficiency of adult pancreatic islets in the treatment of diabetic patients. Methods Thirteen adult donor panceata were used for this study. After collagenase digestion, the islets were isolated and purified followed by transplantation into diabetes SPF nude mice. Control group were transplanted by normal saline solution. Islet graft survival was monitored by the detection of glucose changes in diabetic mice and histological examination. Results Islet yield was (3608±403) IEQ/g in this study, while that was (2820±318)IEQ/g after purification with a purity of 85%. The blood glucose of 84.6% diabetic mice decreased to normal in the first day post-transplantation. Normal blood glucose level maintained for a long time. There was significant difference in the Kaplan-Meier analysis for the islet survival (P =0.0012). Conclusion The adult donor pancreas is an ideal source for islet transplantation due to high yield and good function with a high purity and viability to correct the diabetic nude mice blood glucose level.