CAJ | 학술논문

应用蛋白质胶条鉴定技术，对DB101在接种后应对青枯病菌入侵时部分蛋白质组分的变化过程进行了探索。发现分子量在50～60 kD之间的部分蛋白质组分在该品种发病前后发生显著变化，进行生物信息学分析，得到2个与防御机制相关的蛋白。这些蛋白主要存在于组成剪切体的细胞核核糖核蛋白的合成和代谢通路中，和ABC转运子的合成与代谢也有关系。
응용단백질효조감정기술，대DB101재접충후응대청고병균입침시부분단백질조분적변화과정진행료탐색。발현분자량재50～60 kD지간적부분단백질조분재해품충발병전후발생현저변화，진행생물신식학분석，득도2개여방어궤제상관적단백。저사단백주요존재우조성전절체적세포핵핵당핵단백적합성화대사통로중，화ABC전운자적합성여대사야유관계。
DB101 is a tobacco variety developed in the United States with resistance to bacterial wilt, usually used for resistant control in identification of tobacco bacterial wilt disease. Understanding its resistance mechanism to bacterial wilt provide a theoretical basis for choosing breeding material. Proteomics study is a very important way to understand crop resistance mechanism to pathogenic microorganisms. In this experiment, protein strip identification technique was used to explore response to invasion of Ralstonia solanacearum of DB101 by studying process change of some protein components after vaccination. Results showed that protein components weight between 50~60kD got a significant change before and after onset of the variety. After bioinformatics analysis, two proteins were identified being related to defense mechanisms of this plant. These proteins were found primarily in synthesis and metabolism pathways of ABC transporter and cell nuclear ribonucleoprotein constructing spliced variant.