CAJ | 학술논문

目的：建立一种能够快速、准确地检测流感病毒亚单位疫苗中间体样品中沙门菌污染的方法。方法：首先利用选择性增菌培养基对样品进行增菌培养，然后提取样品中的细菌基因组DNA，通过沙门菌特异性引物对基因组DNA进行PCR扩增，以琼脂糖凝胶电泳实现对目的片段的检测。结果：该PCR反应体系的扩增检测灵敏度可达1 pg的沙门菌DNA，利用选择性增菌培养配合该PCR体系可在最快24 h内实现对沙门菌的准确检测，测定结果与传统方法相符。结论：此方法应用于流感病毒亚单位疫苗中间体的沙门菌检查，较之传统的培养法结合生化鉴定的方法，大大缩短了检测周期，降低了结果判读的难度，在实际生产中有很好的应用前景。
목적：건립일충능구쾌속、준학지검측류감병독아단위역묘중간체양품중사문균오염적방법。방법：수선이용선택성증균배양기대양품진행증균배양，연후제취양품중적세균기인조DNA，통과사문균특이성인물대기인조DNA진행PCR확증，이경지당응효전영실현대목적편단적검측。결과：해PCR반응체계적확증검측령민도가체1 pg적사문균DNA，이용선택성증균배양배합해PCR체계가재최쾌24 h내실현대사문균적준학검측，측정결과여전통방법상부。결론：차방법응용우류감병독아단위역묘중간체적사문균검사，교지전통적배양법결합생화감정적방법，대대축단료검측주기，강저료결과판독적난도，재실제생산중유흔호적응용전경。
Objective: To establish a rapid and accurate detection for Salmonella in influenza virus subunit vac?cine intermediates. Methods: Sample genomic DNA was extracted after cultivation in enrichment media, target seg?ment was amplified by specific primers thereafter, and detected by agarose gel electrophoresis. Results: Sensitivity of the PCR assay was 1 pg Salmonella DNA. Detection assay could be accomplished properly in 24 hours by se?lective enrichment cultures and PCR, the results was in accord with traditional method. Conclusion: This method provided a faster and unambiguous resolution in Salmonella detection of influenza subunit vaccine intermediate, in comparison of traditional method of cultivation accompanied by biochemical assay, and has good application pros?pects in vaccine production.