CAJ | 학술논문

由烟草普通花叶病毒（Tobacco mosaic virus, TMV）引起的病毒病是烟草上发病早、传播快的主要病害之一，农事操作机械接触传染及土壤病残传毒是TMV的主要流行因子。为应用生防菌进行土壤和工具消毒，对已筛选的5株生防菌（Ba、CZ、A3、4A1、2A2），将其单菌株培养液及菌株两两混合接种后的培养液与TMV等体积混合，采用摩擦接种枯斑寄主三生NN烟的侵染力测定法进行菌株活性比较。结果显示，5株生防菌培养液及两两混合接种后的培养液对TMV均有较强的抑制活性，与NB培养基对照相比，其5倍稀释液对TMV的抑制效果为94%~99%，以CZ+2A2混合接种培养液对TMV抑制效果最好，50倍稀释液防效为74.9％。具备单独或混合培养制备土壤消毒剂用以工具及苗床和大田土壤消毒的潜力。
유연초보통화협병독（Tobacco mosaic virus, TMV）인기적병독병시연초상발병조、전파쾌적주요병해지일，농사조작궤계접촉전염급토양병잔전독시TMV적주요류행인자。위응용생방균진행토양화공구소독，대이사선적5주생방균（Ba、CZ、A3、4A1、2A2），장기단균주배양액급균주량량혼합접충후적배양액여TMV등체적혼합，채용마찰접충고반기주삼생NN연적침염력측정법진행균주활성비교。결과현시，5주생방균배양액급량량혼합접충후적배양액대TMV균유교강적억제활성，여NB배양기대조상비，기5배희석액대TMV적억제효과위94%~99%，이CZ+2A2혼합접충배양액대TMV억제효과최호，50배희석액방효위74.9％。구비단독혹혼합배양제비토양소독제용이공구급묘상화대전토양소독적잠력。
Tobacco mosaic virus (TMV) is one of the major diseases on tobacco, spreading fast and popular early. Mechanical contact infection in farm work and transmission of virus residues in soils are the major culprits for the rampant outbreak of TMV. For application of bio-control bacteria in soil and instruments disinfection, the suppression in vitro of the five single bacteria cultures and the twain bacteria cultures on TMV was investigated on local lesion host of Nicotiana tabacum var. samsum NN challenged by mechanical inoculation of mixture of cultures with TMV. The results showed that both of the five single bacteria cultures and the twain bacteria cultures had good disinfection effect on TMV, all of 5-fold dilution cultures suppressed TMV from 94%to 99%in vitro compared to the mixture of NB with TMV. And the twain bacteria cultures of CZ and 2A2 had the best disinfection effect, with 74.90% of suppression in vitro by 50-fold dilution cultures. These results indicated that CZ and 2A2 has a potential to be an instruments and soils disinfector against TMV.