CAJ | 학술논문

日照长度影响植物的生长发育，在拟南芥中CONSTANS是植物光周期开花途径中的关键基因。利用同源序列法结合 RACE 技术从短日照烟草品种 Kutsaga.Mammoth10中分离出了开花时间相关的 CO（CONSTANS）同源基因，并命名为NtCO1（基因登录号JN022535.1）。经序列分析，NtCO1全长1493 bp，具有完整的开放阅读框（ORF，81~1292 bp），编码403个氨基酸；具有CO蛋白典型的结构域；氨基末端有两个B-box结构，羧基末端有CCT保守结构域。氨基酸同源性比对发现，NtCO1与茄科CO同源蛋白一致性最高，同马铃薯CO序列一致性达到86.5%；与拟南芥CO蛋白和水稻Hd1氨基酸序列一致性也分别达到50%和43.7%。基因表达分析表明，NtCO1在叶片中优势表达，茎中次之，根中最弱。
일조장도영향식물적생장발육，재의남개중CONSTANS시식물광주기개화도경중적관건기인。이용동원서렬법결합 RACE 기술종단일조연초품충 Kutsaga.Mammoth10중분리출료개화시간상관적 CO（CONSTANS）동원기인，병명명위NtCO1（기인등록호JN022535.1）。경서렬분석，NtCO1전장1493 bp，구유완정적개방열독광（ORF，81~1292 bp），편마403개안기산；구유CO단백전형적결구역；안기말단유량개B-box결구，최기말단유CCT보수결구역。안기산동원성비대발현，NtCO1여가과CO동원단백일치성최고，동마령서CO서렬일치성체도86.5%；여의남개CO단백화수도Hd1안기산서렬일치성야분별체도50%화43.7%。기인표체분석표명，NtCO1재협편중우세표체，경중차지，근중최약。
Day length controls development in many plants. In Arabidopsis thaliana, the CONSTANS (CO) gene is a key component in the photoperiodic pathway controlling floral transition. A novel cDNA encoding a CO homolog was isolated from Nicotiana tabacum cv. Kutsaga Mammoth 10 and designated NtCO1 (GenBank accession number, JN022535.1). The full cDNA sequence was 1493 bp with an open reading frame (ORF) of 1211 bp, encoding a protein of 403 amino acids. The predicted NtCO1 protein contained two B-box-type zinc fingers and a CCT domain. Analysis based on amino acid sequence alignment showed that NtCO1 shared high identity with StCO (86.5%) from Solanum tuberosum, AtCO (50%) from A.thaliana and Hd1 (43.7%) from Oryza sativa. Semi-quantitative RT-PCR analysis showed that NtCO1 was expressed specifically and strongly in leaf tissues but weakly in stem and root tissues.