中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2013年
3期
55-59
,共5页
文轲%张志明%任民%蒋彩虹%申莉莉%程立锐%耿锐梅%陈小翠%冯莉%杨爱国
文軻%張誌明%任民%蔣綵虹%申莉莉%程立銳%耿銳梅%陳小翠%馮莉%楊愛國
문가%장지명%임민%장채홍%신리리%정립예%경예매%진소취%풍리%양애국
烟草%CMV%SSR%抗性基因
煙草%CMV%SSR%抗性基因
연초%CMV%SSR%항성기인
tobacco%CMV%SSR%resistance gene
利用 SSR 技术，在抗 CMV 烟草中发掘与抗病基因紧密连锁的分子标记，目的是为抗病基因的定位克隆以及培育抗病新品种奠定基础。本研究以抗CMV的烤烟品种台烟8号、感病品种NC82为亲本，构建BC1代分离群体，对其进行抗性遗传分析。对分布于烟草24条连锁群上的2317个SSR位点进行了多态性筛选，获得了62个稳定多态性标记，利用这些标记对288株BC1群体分型数据，用WinQTLCart2.5进行分析，找到一个与抗性相关的QTL，LOD值为2.6。研究结论表明，台烟8号对CMV的抗性由多基因控制，标记53735与抗性QTL紧密连锁，遗传距离为0.1 cM，该QTL位于10号连锁群，贡献率为13%。
利用 SSR 技術，在抗 CMV 煙草中髮掘與抗病基因緊密連鎖的分子標記，目的是為抗病基因的定位剋隆以及培育抗病新品種奠定基礎。本研究以抗CMV的烤煙品種檯煙8號、感病品種NC82為親本，構建BC1代分離群體，對其進行抗性遺傳分析。對分佈于煙草24條連鎖群上的2317箇SSR位點進行瞭多態性篩選，穫得瞭62箇穩定多態性標記，利用這些標記對288株BC1群體分型數據，用WinQTLCart2.5進行分析，找到一箇與抗性相關的QTL，LOD值為2.6。研究結論錶明，檯煙8號對CMV的抗性由多基因控製，標記53735與抗性QTL緊密連鎖，遺傳距離為0.1 cM，該QTL位于10號連鎖群，貢獻率為13%。
이용 SSR 기술，재항 CMV 연초중발굴여항병기인긴밀련쇄적분자표기，목적시위항병기인적정위극륭이급배육항병신품충전정기출。본연구이항CMV적고연품충태연8호、감병품충NC82위친본，구건BC1대분리군체，대기진행항성유전분석。대분포우연초24조련쇄군상적2317개SSR위점진행료다태성사선，획득료62개은정다태성표기，이용저사표기대288주BC1군체분형수거，용WinQTLCart2.5진행분석，조도일개여항성상관적QTL，LOD치위2.6。연구결론표명，태연8호대CMV적항성유다기인공제，표기53735여항성QTL긴밀련쇄，유전거리위0.1 cM，해QTL위우10호련쇄군，공헌솔위13%。
In order to obtian gene closely linked with CMV and cultivate resistant varieties, BC1 segregating population was generated from Taiyan-8 (resistant) and NC82 (susceptible) as parents. The BC1 population was used for virus artificial inoculation. 167 polymorphism markers were selected from 2317 SSR markers distributed in 24 tobacco linkage populations and then 62 SSR markers with stability and significant polymorphism were obtained. A tobacco genetic linkage map was constructed according to virus test results of 288 BC1. A QTL related to resistance with LOD equal to 2.6 was obtianed through WinQTLCart2.5 analysis. Our results showed that multiple genes caused the resistance of Taiyan-8;marker 53735 was closely linked to resistance QTL with the genetic distance of 0.1 cM, which could explain 13%of the variation.
