CAJ | 학술논문

线粒体基因异常可能导致烟草细胞质雄性不育。sdh3、sdh4是编码线粒体电子传递链复合体 II 亚基的重要基因，为探讨线粒体基因sdh3、sdh4与细胞质雄性不育的关系，从3个普通烟草不育系品种及其保持系中提取线粒体DNA，根据GenBank报道的烟草线粒体DNA序列设计特异引物扩增目的基因并克隆测序，应用生物信息学方法，分析烟草雄性不育系及保持系的sdh3、sdh4基因在其核苷酸、编码蛋白各结构层次上存在的差异。结果显示，不育系的sdh4基因第28位碱基发生了改变；不育系的sdh3基因发生10个碱基的缺失，以致其编码蛋白改变，这极可能成为干扰线粒体琥珀酸脱氢酶亚基3功能的关键因素，从而成为可能导致烟草CMS的根本原因之一。
선립체기인이상가능도치연초세포질웅성불육。sdh3、sdh4시편마선립체전자전체련복합체 II 아기적중요기인，위탐토선립체기인sdh3、sdh4여세포질웅성불육적관계，종3개보통연초불육계품충급기보지계중제취선립체DNA，근거GenBank보도적연초선립체DNA서렬설계특이인물확증목적기인병극륭측서，응용생물신식학방법，분석연초웅성불육계급보지계적sdh3、sdh4기인재기핵감산、편마단백각결구층차상존재적차이。결과현시，불육계적sdh4기인제28위감기발생료개변；불육계적sdh3기인발생10개감기적결실，이치기편마단백개변，저겁가능성위간우선립체호박산탈경매아기3공능적관건인소，종이성위가능도치연초CMS적근본원인지일。
The abnormal mitochondrial gene may lead to tobacco cytoplasmic male sterility. sdh3 and sdh4 are important genes coding subunits of mitochondrial complex II. To study the relationship between mitochondrial genes sdh3, sdh4 and cytoplasmic male sterility, the mitochondrial DNA from 3 male sterile lines and their maintainer lines of Nicotiana tabacum cultivars were extracted, specific primers were designed according to the complete nucleotide sequence of the tobacco mitochondrial genome in GenBank to amplify purpose genes from tobacco mitochondrial DNA. PCR products were cloned and sequenced. The sdh3 and sdh4 nucleotides and encoded protein structures were analyzed with bioinformatics to find differences between male sterile lines and maintainer lines. The results showed that the 28th base of sdh4 of male sterile lines gene changed. The sdh3 of male sterile lines gene lost 10 bases, and lead to the encoded protein altered, this is much more likely to be the key factors interference with the function of mitochondrial succinic dehydrogenase subunit 3, and one of root factors generated tobacco CMS.