基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2013年
3期
409-412
,共4页
神秘果%果实%RNA提取
神祕果%果實%RNA提取
신비과%과실%RNA제취
Miracle fruit%Fruit%RNA isolation
以神秘果果实为材料，以获得高质量RNA为目的，以针对性地去除多糖、酚类物质为出发点，比较了Trizol法、CTAB法，并相应加以改良。结果显示：CTAB粗提法方便快捷，CTAB精提法提取的总RNA完整性好、纯度高，RT-PCR反应表明提取的总RNA都能满足分子生物学实验的要求。
以神祕果果實為材料，以穫得高質量RNA為目的，以針對性地去除多糖、酚類物質為齣髮點，比較瞭Trizol法、CTAB法，併相應加以改良。結果顯示：CTAB粗提法方便快捷，CTAB精提法提取的總RNA完整性好、純度高，RT-PCR反應錶明提取的總RNA都能滿足分子生物學實驗的要求。
이신비과과실위재료，이획득고질량RNA위목적，이침대성지거제다당、분류물질위출발점，비교료Trizol법、CTAB법，병상응가이개량。결과현시：CTAB조제법방편쾌첩，CTAB정제법제취적총RNA완정성호、순도고，RT-PCR반응표명제취적총RNA도능만족분자생물학실험적요구。
In order to extract total RNA form miracle fruit and reduce the influence of polysaccharides and polyphenolics, we studied and compared four RNA extraction methods such as Trizol method, Trizol improved method, CTAB crude method and CTAB refined method. The results showed that: The CTAB crude extraction method was convenient and quick, total RAN from the CTAB refined method was proved to be relatively intact and high purity, RT-PCR analysis showed that the total RNA from both CTAB methods could be used in further molecular biology research.
