CAJ | 학술논문

为了确定稻属AA基因组物种间的遗传差异和系统进化关系，62份来自广泛地理分布的水稻品系被用于 ISSR 标记分析。这些品系包含有6个野生稻种（O. nivara, O. rufipogon、O. barthi, O. longistaminata, O. glumaepatula,和O. meridionalis）和2个栽培稻种(O. sativa 和O. glaberrima)。21条能产生良好重复性条带模式的ISSR引物被筛选出，并在62个水稻品系中揭示出非常好的多态性。全部样品的基因多样性为0.527，同时显示出ISSR标记在稻属物种遗传多样性研究中具有强大的作用。根据ISSR条带模式，利用Jaccard配对相似系数构建的一致性树状图，显示出具有良好自展支持率的稻属AA基因组遗传多样性关系。结果表明，来自不同大陆的稻属物种具有较近的亲缘关系，尤其是亚洲野生稻物种与Vaughan1989年建立的稻属分类系统具有良好的一致性。研究结果将对稻属AA基因组野生稻在水稻育种实践中的有效利用具有非常重要的意义。
위료학정도속AA기인조물충간적유전차이화계통진화관계，62빈래자엄범지리분포적수도품계피용우 ISSR 표기분석。저사품계포함유6개야생도충（O. nivara, O. rufipogon、O. barthi, O. longistaminata, O. glumaepatula,화O. meridionalis）화2개재배도충(O. sativa 화O. glaberrima)。21조능산생량호중복성조대모식적ISSR인물피사선출，병재62개수도품계중게시출비상호적다태성。전부양품적기인다양성위0.527，동시현시출ISSR표기재도속물충유전다양성연구중구유강대적작용。근거ISSR조대모식，이용Jaccard배대상사계수구건적일치성수상도，현시출구유량호자전지지솔적도속AA기인조유전다양성관계。결과표명，래자불동대륙적도속물충구유교근적친연관계，우기시아주야생도물충여Vaughan1989년건립적도속분류계통구유량호적일치성。연구결과장대도속AA기인조야생도재수도육충실천중적유효이용구유비상중요적의의。
In order to determine genetic diversity of the AA-genome Oryza species (Poaceae), inter-simple sequence repeat (ISSR) markers from a total of 62 rice accessions collected worldwide were analyzed. These accessions encompassed six wild (O. nivara, O. rufipogon, O. barthii, O. longistaminata, O. glumaepatula, and O. meridionalis) and two cultivated (O. sativa and O. glaberrima) species. 21 selected ISSR primers that produced consistent and repeatable banding patterns revealed significant polymorphisms among the 62 rice accessions with an overall gene diversity (DG) of 0.527, indicating the power of ISSR markers in studying genetic diversity in Oryza germplasm. The consensus tree constructed on the basis of the pairwise Jaccard similarity coefficients of the ISSR banding pattern revealed an evident genetic variation relationships of the AA-genome Oryza species with high bootstrap value supports. It is concluded from this study that the Oryza species from different continents possessed close linkages and current classification of the AA-genome Oryza species suggested by Vaughan (1989) remains valid, particularly in relation to that of the Asian wild rice. The knowledge will be useful for the effective utilization of AA-genome wild Oryza species in rice breeding programs.