CAJ | 학술논문

对实验室保存的一株产α-淀粉酶的枯草芽孢杆菌的16S rDNA 区进行克隆及序列分析。采用 PCR 克隆方法，对其菌株的16S rDNA 区进行序列扩增，扩增产物经琼脂糖凝胶电泳，获得一个大小约为1300 bp 的特异性扩增条带，随后将测序结果用 GeneBank 数据库中的 BLAST 软件与获取的已知枯草芽孢杆菌的16S rDNA 序列进行序列比对分析。结果表明：该枯草芽孢杆菌与枯草芽孢杆菌 FZB42具有相似的序列，相似性为97%。根据上述分析结果可判定2种枯草芽孢杆菌为同属细菌。
대실험실보존적일주산α-정분매적고초아포간균적16S rDNA 구진행극륭급서렬분석。채용 PCR 극륭방법，대기균주적16S rDNA 구진행서렬확증，확증산물경경지당응효전영，획득일개대소약위1300 bp 적특이성확증조대，수후장측서결과용 GeneBank 수거고중적 BLAST 연건여획취적이지고초아포간균적16S rDNA 서렬진행서렬비대분석。결과표명：해고초아포간균여고초아포간균 FZB42구유상사적서렬，상사성위97%。근거상술분석결과가판정2충고초아포간균위동속세균。
This experiment adopted the genome which extracted form Bacillus subtilis strain that can hydrolyze amylase as a template,used the PCR cloning method to amplify the 16S rDNA Regions sequence of it.Through the agarose gel electrophoresis of the PCR products,a specific amplification bands in the size of approximately 1 300 bp was got.Used the sequence determination and the comparative analysis of the known Bacillus subtilis's 16S rDNA sequence which got from the International Molecular Biology Database in Internet,the results showed that,according to the results of the 16S rDNA sequence analysis,it would be found that the similarity between the two strains was about 97%.It can be initially identified that these two strains belong to the same kind.