CAJ | 학술논문

@@@@对培养基的无机盐浓度、钾浓度比例、氮素型态、植物生长调节剂进行了试验优化，研究了培养基和外植体取材对蝴蝶兰茎尖生长点培养脱病毒的影响。结果表明：经MS培养基改良的KN培养基，其1/5大量元素的无机盐浓度适合蝴蝶兰茎尖生长点的培养。一定浓度范围内，提高氮源中硝酸态氮的比例，或提高培养基中钾的浓度比例，均有助于蝴蝶兰茎尖生长点培养的存活。植物生长调节剂NAA 0.5 mg·L-1+6-BA 3.5 mg·L-1的组合适合蝴蝶兰茎尖生长点培养的存活和类原球茎(Protocorm-Like Body,PLBs)的诱导。茎尖取材大利于培养存活，但是不利于脱病毒效果。齿舌兰轮斑病毒(简称ORSV)的茎尖生长点脱病毒难度较建兰花叶病毒(简称CymMV)的脱病毒难度大。蝴蝶兰茎尖培养脱病毒取材外植体须小于0.4 mm，附带叶原基不能多于1个。
@@@@대배양기적무궤염농도、갑농도비례、담소형태、식물생장조절제진행료시험우화，연구료배양기화외식체취재대호접란경첨생장점배양탈병독적영향。결과표명：경MS배양기개량적KN배양기，기1/5대량원소적무궤염농도괄합호접란경첨생장점적배양。일정농도범위내，제고담원중초산태담적비례，혹제고배양기중갑적농도비례，균유조우호접란경첨생장점배양적존활。식물생장조절제NAA 0.5 mg·L-1+6-BA 3.5 mg·L-1적조합괄합호접란경첨생장점배양적존활화류원구경(Protocorm-Like Body,PLBs)적유도。경첨취재대리우배양존활，단시불리우탈병독효과。치설란륜반병독(간칭ORSV)적경첨생장점탈병독난도교건란화협병독(간칭CymMV)적탈병독난도대。호접란경첨배양탈병독취재외식체수소우0.4 mm，부대협원기불능다우1개。
The effect of culture medium and explant on the tip-shoot tissue culture for eradicating orchid virus was studied. The concentrations of inorganic salt, nitrogen, potassium, and plant growth regulator in culture medium were tested and optimized. The results showed that 1/5 inorganic salt quantity of KN medium improved from the MS medium is suitable to tip-shoot culture of Phalaenopsis. Within a certain concentration range, increasing the proportion of nitrate nitrogen in total nitrogen content and the potassium concentration proportion in total inorganic salt of medium contribute to improve the survival of tip-shoot culture. The plant growth regulator combination of NAA 0.5 mg·L-1+6-BA 3.5 mg·L-1 is suitable to the Protocorm Like Bodies (PLBs) inducted from the Phalaenopsis tip-shoot. Big drawn size of tip-shoot organization is conductive to the survival of tip-shoot culture but detrimental to virus-eradicting effect. The orchid virus ORSV is more difficult to deal with than the orchid CymMV. Drawn size smaller than 0.4 mm, attaching leaf primordium no more than one is necessary for thorough eradication of orchid virus by the way of tip-shoot culture.