中国园艺文摘
中國園藝文摘
중국완예문적
CHINESE HORTICULTURE ABSTRACTS
2013年
4期
7-9,64
,共4页
付琬%辛学锐%金郁%王火旭
付琬%辛學銳%金鬱%王火旭
부완%신학예%금욱%왕화욱
番茄%番茄黄化曲叶病毒(TYLCV)抗性基因%多重PCR%分子标记
番茄%番茄黃化麯葉病毒(TYLCV)抗性基因%多重PCR%分子標記
번가%번가황화곡협병독(TYLCV)항성기인%다중PCR%분자표기
tomato%TYLCV resistant gene%multiplex-PCR%molecular marker
@@@@试验探索在同一个反应体系中完成3个SCAR标记的多重PCR扩增，为利用分子标记辅助番茄黄化曲叶病抗性育种提供一种省时、省力、经济有效的方法。对分别与番茄黄化曲叶病毒病抗性基因 Ty-1、Ty-2、Ty-3紧密连锁的共显性SCAR标记同时进行扩增筛选，扩增的特异性片段与单引物扩增片段一致。
@@@@試驗探索在同一箇反應體繫中完成3箇SCAR標記的多重PCR擴增，為利用分子標記輔助番茄黃化麯葉病抗性育種提供一種省時、省力、經濟有效的方法。對分彆與番茄黃化麯葉病毒病抗性基因 Ty-1、Ty-2、Ty-3緊密連鎖的共顯性SCAR標記同時進行擴增篩選，擴增的特異性片段與單引物擴增片段一緻。
@@@@시험탐색재동일개반응체계중완성3개SCAR표기적다중PCR확증，위이용분자표기보조번가황화곡협병항성육충제공일충성시、성력、경제유효적방법。대분별여번가황화곡협병독병항성기인 Ty-1、Ty-2、Ty-3긴밀련쇄적공현성SCAR표기동시진행확증사선，확증적특이성편단여단인물확증편단일치。
The experiment explored a method of an mutiplex PCR amplification of three SCAR markers in one reaction system and to provide a time saving, economical and effective method for tomato yellow leaf curl virus (TYLCV) resistance breeding by molecular marker-assisted selection. Three tomato co-dominant SCAR markers tightly linked respectively with TYLCV resistant gene Ty-1, Ty-2 or Ty-3 were amplified in one reaction system.The PCR products were completely correspond to the bands amplified by single SCAR primer pair.
