国际口腔医学杂志
國際口腔醫學雜誌
국제구강의학잡지
JOURNAL OF INTERNATIONAL STOMATOLOGY
2013年
4期
429-431
,共3页
陈意%陶人川%林雪芳%刘贞敏%雍翔智%李润英
陳意%陶人川%林雪芳%劉貞敏%雍翔智%李潤英
진의%도인천%림설방%류정민%옹상지%리윤영
日节律性%人中性粒细胞防御素%龈沟液%外周血液
日節律性%人中性粒細胞防禦素%齦溝液%外週血液
일절률성%인중성립세포방어소%간구액%외주혈액
circadian rhythm%human neutrophil peptide%gingival crevicular fluid%peripheral venous blood
目的探索人中性粒细胞防御素1~3(HNP1~3)表达水平的日节律性,为其作为生物学指标提供科学依据。方法对6名健康志愿者,连续2 d从6时至22时定点采集唾液、外周血及4颗第一恒磨牙近颊沟处的龈沟液,运用酶联免疫吸附测定(ELISA)方法检测HNP1~3的表达量。结果6名受试者HNP1~3的表达量和变化趋势为:龈沟液的中位数为5.86μg·L-1,总体变化趋势为正弦波形,14时最高,18时最低;唾液的中位数为2.15μg·L-1,总体变化趋势为“V”字波形,早晚较高,14时达峰谷;血液的中位数为4.79μg·L-1,总体变化趋势为单峰波形,6时至10时呈上升趋势并达峰值,继而缓慢下降。结论在3种体液中, HNP1~3表达量存在差异性,其昼夜的表达呈现一定节律性。
目的探索人中性粒細胞防禦素1~3(HNP1~3)錶達水平的日節律性,為其作為生物學指標提供科學依據。方法對6名健康誌願者,連續2 d從6時至22時定點採集唾液、外週血及4顆第一恆磨牙近頰溝處的齦溝液,運用酶聯免疫吸附測定(ELISA)方法檢測HNP1~3的錶達量。結果6名受試者HNP1~3的錶達量和變化趨勢為:齦溝液的中位數為5.86μg·L-1,總體變化趨勢為正絃波形,14時最高,18時最低;唾液的中位數為2.15μg·L-1,總體變化趨勢為“V”字波形,早晚較高,14時達峰穀;血液的中位數為4.79μg·L-1,總體變化趨勢為單峰波形,6時至10時呈上升趨勢併達峰值,繼而緩慢下降。結論在3種體液中, HNP1~3錶達量存在差異性,其晝夜的錶達呈現一定節律性。
목적탐색인중성립세포방어소1~3(HNP1~3)표체수평적일절률성,위기작위생물학지표제공과학의거。방법대6명건강지원자,련속2 d종6시지22시정점채집타액、외주혈급4과제일항마아근협구처적간구액,운용매련면역흡부측정(ELISA)방법검측HNP1~3적표체량。결과6명수시자HNP1~3적표체량화변화추세위:간구액적중위수위5.86μg·L-1,총체변화추세위정현파형,14시최고,18시최저;타액적중위수위2.15μg·L-1,총체변화추세위“V”자파형,조만교고,14시체봉곡;혈액적중위수위4.79μg·L-1,총체변화추세위단봉파형,6시지10시정상승추세병체봉치,계이완만하강。결론재3충체액중, HNP1~3표체량존재차이성,기주야적표체정현일정절률성。
Objective To detect the human neutrophil peptide 1-3(HNP1-3) expression in circadian rhythm. Methods Saliva, peripheral venous blood and gingival crevicular fluid(GCF) samples from mesio-buccal gingival crevice of four first molars of six healthy individuals were collected at two consecutive days from 6∶00 to 22∶00, and the expression of HNP1-3 was detected by enzyme-linked immunosorbent assay(ELISA) method. Results The median in GCF was 5.86μg·L-1, the overall trend was a sinusoidal waveform, with peak at 14∶00 and valley at 20∶00. The median in saliva was 2.15μg·L-1, the overall trend was the “V” word waveform, higher in the morning and evening, the valley at 14∶00. The median in peripheral venous blood was 4.79μg·L-1, the overall trend was single peak waveform, reach-ing a peak from 6∶00 to 10∶00, and then declining slowly. Conclusion The expression HNP1-3 in saliva, GCF, pe-ripheral venous blood samples existed differences. The trend from high to low was GCF, blood, saliva and showed a certain circadian rhythm.