CAJ | 학술논문

目的探索人中性粒细胞防御素1~3（HNP1~3）表达水平的日节律性，为其作为生物学指标提供科学依据。方法对6名健康志愿者，连续2 d从6时至22时定点采集唾液、外周血及4颗第一恒磨牙近颊沟处的龈沟液，运用酶联免疫吸附测定（ELISA）方法检测HNP1～3的表达量。结果6名受试者HNP1~3的表达量和变化趋势为：龈沟液的中位数为5.86μg·L-1，总体变化趋势为正弦波形，14时最高，18时最低；唾液的中位数为2.15μg·L-1，总体变化趋势为“V”字波形，早晚较高，14时达峰谷；血液的中位数为4.79μg·L-1，总体变化趋势为单峰波形，6时至10时呈上升趋势并达峰值，继而缓慢下降。结论在3种体液中， HNP1~3表达量存在差异性，其昼夜的表达呈现一定节律性。
목적탐색인중성립세포방어소1~3（HNP1~3）표체수평적일절률성，위기작위생물학지표제공과학의거。방법대6명건강지원자，련속2 d종6시지22시정점채집타액、외주혈급4과제일항마아근협구처적간구액，운용매련면역흡부측정（ELISA）방법검측HNP1～3적표체량。결과6명수시자HNP1~3적표체량화변화추세위：간구액적중위수위5.86μg·L-1，총체변화추세위정현파형，14시최고，18시최저；타액적중위수위2.15μg·L-1，총체변화추세위“V”자파형，조만교고，14시체봉곡；혈액적중위수위4.79μg·L-1，총체변화추세위단봉파형，6시지10시정상승추세병체봉치，계이완만하강。결론재3충체액중， HNP1~3표체량존재차이성，기주야적표체정현일정절률성。
Objective To detect the human neutrophil peptide 1-3(HNP1-3) expression in circadian rhythm. Methods Saliva, peripheral venous blood and gingival crevicular fluid(GCF) samples from mesio-buccal gingival crevice of four first molars of six healthy individuals were collected at two consecutive days from 6∶00 to 22∶00, and the expression of HNP1-3 was detected by enzyme-linked immunosorbent assay(ELISA) method. Results The median in GCF was 5.86μg·L-1, the overall trend was a sinusoidal waveform, with peak at 14∶00 and valley at 20∶00. The median in saliva was 2.15μg·L-1, the overall trend was the “V” word waveform, higher in the morning and evening, the valley at 14∶00. The median in peripheral venous blood was 4.79μg·L-1, the overall trend was single peak waveform, reach-ing a peak from 6∶00 to 10∶00, and then declining slowly. Conclusion The expression HNP1-3 in saliva, GCF, pe-ripheral venous blood samples existed differences. The trend from high to low was GCF, blood, saliva and showed a certain circadian rhythm.