CAJ | 학술논문

以不同浓度的农药吡虫啉溶液为染毒药物，以牛蛙为实验对象，研究了牛蛙在0．005～0．04 g/L吡虫啉溶液的胁迫下，牛蛙外周血中血红细胞数、红细胞微核率、核异常率以及总核异常率的变化，以期发挥牛蛙在环境污染生物监测中的作用.结果表明：吡虫啉对牛蛙血红细胞数、红细胞微核率、核异常率以及总核异常率均有一定的影响.吡虫啉浓度为0．04 g/L时，染毒组的血红细胞数不仅低于对照组，而且达到了差异显著水平（p<0．05）或差异极显著水平（p<0．01）；吡虫啉浓度≥0．02 g/L时，所有染毒组的血红细胞微核率不仅高于对照组，而且均达到了差异显著水平（p<0．05）或差异极显著水平（p<0．01或p<0．001）；当吡虫啉浓度≥0．01 g/L时，所有染毒组的血红细胞核异常率及总核异常率均高于对照组，而且均达到了差异极显著水平（p<0．01或p<0．001）.
이불동농도적농약필충람용액위염독약물，이우와위실험대상，연구료우와재0．005～0．04 g/L필충람용액적협박하，우와외주혈중혈홍세포수、홍세포미핵솔、핵이상솔이급총핵이상솔적변화，이기발휘우와재배경오염생물감측중적작용.결과표명：필충람대우와혈홍세포수、홍세포미핵솔、핵이상솔이급총핵이상솔균유일정적영향.필충람농도위0．04 g/L시，염독조적혈홍세포수불부저우대조조，이차체도료차이현저수평（p<0．05）혹차이겁현저수평（p<0．01）；필충람농도≥0．02 g/L시，소유염독조적혈홍세포미핵솔불부고우대조조，이차균체도료차이현저수평（p<0．05）혹차이겁현저수평（p<0．01혹p<0．001）；당필충람농도≥0．01 g/L시，소유염독조적혈홍세포핵이상솔급총핵이상솔균고우대조조，이차균체도료차이겁현저수평（p<0．01혹p<0．001）.
The pesticide(imidacloprid) solution was taken as the target poison and bullfrog(Rana catesbeiana) was taken as the experimental animal. The imidacloprid concentrations were set in the range from 0.005 g/L to 0.04 g/L. The effects of imidacloprid on the numbers,micronuclei rates,nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in bullfrog were studied. The results showed that the imidacloprid could affect the numbers,micronuclei rates,nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in bullfrog. When the concentration of imidacloprid was 0.04 g/L,the numbers of erythrocyte in the experimental groups (treated with imidacloprid) were lower than the number of erythrocyte in the contrast group. And there were significant difference between the experimental groups and contrast group(p<0.05 or p<0.01). When the concentration of imidacloprid ≥0.02 g/L,the micronuclei rates of erythrocyte in the experimental groups were higher than in the contrast group. And the difference between the experimental groups and contrast group were significant(p<0.05, p<0.01 or p<0.001). When the concentration of imidacloprid ≥0.01 g/L,the nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in the experimental groups were higher than in the contrast group too. And there were significant difference between the experimental groups and contrast group(p<0.01 or p<0.001).