CAJ | 학술논문

目的：建立广西产地钱(Marchantia polymorpha L.)拳卷地钱(Marchantia convoluta gao et chang.)和粗裂地钱(Marchantia paleacea Bertol.)的分子鉴别方法。方法：利用 RAPD（random amplified polymorphic DNA）技术。结果：采用改良的 CTAB 法提取地钱、拳卷地钱、粗裂地钱3种植物的总 DNA 均适用于22引物对供试材料 DNA 进行随机扩增，共扩增出145条谱带，多态性条带128条，占扩增总条带的88.3%，平均多态性百分率为89.8%；三种地钱遗传距离为0.3713-0.4107、Nei′s 遗传一致度为0.5893-0.6287。结论：RAPD 技术对广西产地钱、拳卷地钱、粗裂地钱进行分子鉴定是有效的。RAPD 分析。
목적：건립엄서산지전(Marchantia polymorpha L.)권권지전(Marchantia convoluta gao et chang.)화조렬지전(Marchantia paleacea Bertol.)적분자감별방법。방법：이용 RAPD（random amplified polymorphic DNA）기술。결과：채용개량적 CTAB 법제취지전、권권지전、조렬지전3충식물적총 DNA 균괄용우22인물대공시재료 DNA 진행수궤확증，공확증출145조보대，다태성조대128조，점확증총조대적88.3%，평균다태성백분솔위89.8%；삼충지전유전거리위0.3713-0.4107、Nei′s 유전일치도위0.5893-0.6287。결론：RAPD 기술대엄서산지전、권권지전、조렬지전진행분자감정시유효적。RAPD 분석。
Objective: To establish a molecular identification method to identify the Marchantia polymorpha linn. Marchantia convoluta gao et k.c. zhang and Marchantia paleacea bertol. in Guangxi. Methods: the RAPD molecular makers. Results: the modified CTAB method was applicable to analysis the total DNA of the Marchantia polymorpha L.; Marchantia convolute Gao et Chang. and Marchantia paleacea Bert. in Guangxi,145 bands were amplified totally by 22 pair primers, of which 128.Bands (88.3%) were polymorphic and average polymorphism rate was 89.8%; genetic distance was 0.3713-0.4107, Nei's genetic identity was 0.5893-0.6287 Conclusion: The RAPD markers method is available to identify the Marchantia polymorpha linn. Marchantia convoluta gao et k.c. zhang and Marchantia paleacea bertol. in Guangxi