CAJ | 학술논문

Cs－EV12（GenBank 登录号：GH618817）为一受假眼小绿叶蝉取食诱导的茶树香豆酰辅酶 A：莽草酸/奎宁酸香豆酰转移酶的 cDNA 片段，应用 RACE 技术克隆了该基因的全长 cDNA，命名为 CsHCT（GenBank 登录号：JQ619537）。 CsHCT cDNA 序列全长1653 bp，包含一个编码447个氨基酸的完整开放阅读框。序列分析显示 CsH－CT 与毛果杨、烟草、拟南芥的 HCT 在氨基酸序列上一致性分别为54％、41％和40％，含有植物 BAHD 酰基转移酶家族活性中心的保守基序，以及与 HCT 正确折叠有关的 DFGWG 基序。 HCT 的表达受假眼小绿叶蝉取食、低温、紫外线和茉莉酮酸甲酯的诱导。
Cs－EV12（GenBank 등록호：GH618817）위일수가안소록협선취식유도적다수향두선보매 A：망초산/규저산향두선전이매적 cDNA 편단，응용 RACE 기술극륭료해기인적전장 cDNA，명명위 CsHCT（GenBank 등록호：JQ619537）。 CsHCT cDNA 서렬전장1653 bp，포함일개편마447개안기산적완정개방열독광。서렬분석현시 CsH－CT 여모과양、연초、의남개적 HCT 재안기산서렬상일치성분별위54％、41％화40％，함유식물 BAHD 선기전이매가족활성중심적보수기서，이급여 HCT 정학절첩유관적 DFGWG 기서。 HCT 적표체수가안소록협선취식、저온、자외선화말리동산갑지적유도。
@@@@Cs-EV12 is the cDNA fragment of a HCT gene of tea (Camellia sinensis L) up-regulated by mild infestation of green eafhopper (Empoasca vitis G?the).The full-length cDNA of Cs-EV12 was cloned by RACE (rapid amplification of cDNA ends).This cDNA was designated as CsHCT (GenBank accession number: JQ619537), which is 1 653 bp in length, and contains an open reading frame of 447 amino acids.CsHCT protein shares most amino acid sequence in similarity with theHCTs from Populus trichocarpa, Nicotiana tabacum and Arabidopsis thaliana, and the similarities are 54%, 41% and 40%, respectively.CsHCT contains the conserved motif involved in the catalytic activity of BAHD acetyltransferase, and DFGWG motif required for proper folding of the HCT polypeptide.The expression of CsHCT can be induced by leafhopper , feeding, low temperature, UV-B irradiation and methyl jasmonate.