CAJ | 학술논문

利用三维荧光光谱技术研究了定向培育的玉米高产田和对照低产田耕层土壤水溶性有机物在组成和结构上的差异特征。研究结果表明：高产田土壤的有机碳和水溶性有机碳的平均质量分数分别为12．18 g? kg－1和98．68 mg? kg－1，明显高于低产田的9．28 g? kg－1和75．32 mg? kg－1，高产田土壤水溶性有机物的芳香度低于低产田。荧光区域积分结果表明，高产田土壤水溶性有机物的类酪氨酸蛋白质、类色氨酸蛋白质、类溶解性微生物代谢产物的质量分数高于低产田，而类富里酸和类腐殖酸的质量分数则和低产田的基本相同；高产田土壤水溶性有机物中类酪氨酸蛋白质、类溶解性微生物代谢产物所占的比例高于低产田，但类富里酸和类腐殖酸所占的比例稍低于低产田。高产田土壤中微生物源的土壤水溶性有机物量高于低产田。高产田土壤水溶性有机物的类色氨酸蛋白质和类富里酸的芳香度及分子量小于低产田，类富里酸的缩聚度也小于低产田，但类溶解性微生物代谢产物和类腐殖酸的芳香度及分子量大于低产田，类腐殖酸的缩聚度也大于低产田。以上研究结果说明：三维荧光光谱分析技术能够区分开玉米高、低产田土壤耕层的土壤水溶性有机物在组成和结构上的差异，可对玉米高产田的培育研究提供更多有用的信息。
이용삼유형광광보기술연구료정향배육적옥미고산전화대조저산전경층토양수용성유궤물재조성화결구상적차이특정。연구결과표명：고산전토양적유궤탄화수용성유궤탄적평균질량분수분별위12．18 g? kg－1화98．68 mg? kg－1，명현고우저산전적9．28 g? kg－1화75．32 mg? kg－1，고산전토양수용성유궤물적방향도저우저산전。형광구역적분결과표명，고산전토양수용성유궤물적류락안산단백질、류색안산단백질、류용해성미생물대사산물적질량분수고우저산전，이류부리산화류부식산적질량분수칙화저산전적기본상동；고산전토양수용성유궤물중류락안산단백질、류용해성미생물대사산물소점적비례고우저산전，단류부리산화류부식산소점적비례초저우저산전。고산전토양중미생물원적토양수용성유궤물량고우저산전。고산전토양수용성유궤물적류색안산단백질화류부리산적방향도급분자량소우저산전，류부리산적축취도야소우저산전，단류용해성미생물대사산물화류부식산적방향도급분자량대우저산전，류부식산적축취도야대우저산전。이상연구결과설명：삼유형광광보분석기술능구구분개옥미고、저산전토양경층적토양수용성유궤물재조성화결구상적차이，가대옥미고산전적배육연구제공경다유용적신식。
@@@@An experiment was conducted to study the composition and structure of water extractable organic matter (WEOM) be-tween the directive cultivated high yield soil and the controlled low yield soil by three dimensional excitation-emission fluo-rescence spectra.The contents of soil organic carbon and water extractable organic carbon in the high yield soil are 12.18 g? kg-1 and 98.68 mg? kg-1 , respectively, higher than those of 9.28 g? kg-1 and 75.32 mg? kg-1 in low yield soil.The aromaticity of WEOM in high yield soil is higher than that in low yield soil.The results from the fluorescence regional inte-gration show that the contents of tyrosine-like protein, tryptophan-like protein and soluble microbial byproduct-like sub-stance in high yield soil WEOM are higher than those in low yield soil, whereas the contents of fulvic acid-like and humic acid-like organic matter are almost the same in the two kinds of soil WEOM.The percentage of tyrosine-like protein, tryp-tophan-like protein, soluble microbial byproduct-like substance are larger in high yield soil WEOM than those in low yield soil WEOM, while the contents of fulvic acid-like and humic acid-like organic matter are with the controversial trend. Compared to the low yield soil WEOM, the WEOM in high yield soil contains more substance from the activity of microbe. The aromaticity and the molecular weigh of trytophan-like protein and fulvic acid-like substance are lower in high yield soil thanthose in low yield soil.The degree of polycondensation of fulvic acid-like substance in high yield soil is lower than those in low yield soil, whereas the soluble microbial byproduct-like and humicacid -like substance are the opposite. Therefore, three dimensional excitation-emission fluorescence spectra can be used to study the composition and structure of WEOM in soil with different yield and supply more useful information for the cultivation of high yield soil.