CAJ | 학술논문

为获得更多的胞外多糖，采用单因子分析和正交试验相结合的方法，对4个桑黄菌株的液体培养条件进行了优化筛选。结果表明：桑黄4个菌株对营养的需求基本一致，在设置的碳源、氮源和微量元素中，最佳培养基组分为：玉米粉、麸皮、0．2％磷酸二氢钾、0．05％硫酸镁、0．2％碳酸钙、0．05％复合维生素 B 和0．1％维生素E，其中1号、2号菌株液体培养最佳碳氮源质量分数组合为3％玉米粉和2％麸皮；3号、4号菌株为2％玉米粉和2％麸皮。在温度为28℃，初始 pH 值6．50，摇床转速150 r/min 时4个菌株的胞外多糖产量均最高。
위획득경다적포외다당，채용단인자분석화정교시험상결합적방법，대4개상황균주적액체배양조건진행료우화사선。결과표명：상황4개균주대영양적수구기본일치，재설치적탄원、담원화미량원소중，최가배양기조분위：옥미분、부피、0．2％린산이경갑、0．05％류산미、0．2％탄산개、0．05％복합유생소 B 화0．1％유생소E，기중1호、2호균주액체배양최가탄담원질량분수조합위3％옥미분화2％부피；3호、4호균주위2％옥미분화2％부피。재온도위28℃，초시 pH 치6．50，요상전속150 r/min 시4개균주적포외다당산량균최고。
@@@@An experiment was conducted to study the optimal liquid culture conditions of four isolates of Phellinus igniarius to im-prove the production of exocellular polysaccharide by the methods of single factor and orthogonal experiment.The needs of four isolates are basically the same.The most suitable formula of liquid culture medium is as follows: corn meal, wheat bran, 0.2% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate, 0.2% of Calcium Carbonate, 0.05% of compound vitamin B and 0.1% of vitamine E.The best carbon nitrogen source contents for P.igniarius NO.1 and NO.2 are 3% of corn meal and 2% of wheat bran.For P.igniarius NO.3 and NO.4, the carbon nitrogen source contents are 2% of corn meal and 2% of wheat bran.Extracellular polysaccharide yields of the four isolates were the highest under 28℃, 6.50 150 /.