CAJ | 학술논문

以褐藻的主要成分甘露醇为底物，利用丙酮丁醇梭菌 ATCC824发酵生产丁醇。采用 Plackett-Burman（P-B）和 Box-Behnken 优化发酵条件。在 Plackett-Burman 实验设计时，考察牛肉膏、酵母浸粉、胰蛋白胨、乙酸铵、KH2PO4、MgSO4·7H2O、FeSO4·7H2O、接种量、发酵温度、初始 pH 值这10个因素对丁醇产量影响，筛选出影响丁醇发酵的重要参数是发酵温度、初始 pH 值、乙酸铵浓度，利用 Box-Behnken 设计确定最优发酵条件为发酵温度37.3℃、初始 pH 值6.38、乙酸铵浓度2.82 g/L。数学模型分析预测最大丁醇产量为8.47 g/L。经实验验证表明，在优化条件下的丁醇产量平均值达到8.52±0.55 g/L。这说明利用统计学方法优化甘露醇的丁醇发酵条件是有效的。
이갈조적주요성분감로순위저물，이용병동정순사균 ATCC824발효생산정순。채용 Plackett-Burman（P-B）화 Box-Behnken 우화발효조건。재 Plackett-Burman 실험설계시，고찰우육고、효모침분、이단백동、을산안、KH2PO4、MgSO4·7H2O、FeSO4·7H2O、접충량、발효온도、초시 pH 치저10개인소대정순산량영향，사선출영향정순발효적중요삼수시발효온도、초시 pH 치、을산안농도，이용 Box-Behnken 설계학정최우발효조건위발효온도37.3℃、초시 pH 치6.38、을산안농도2.82 g/L。수학모형분석예측최대정순산량위8.47 g/L。경실험험증표명，재우화조건하적정순산량평균치체도8.52±0.55 g/L。저설명이용통계학방법우화감로순적정순발효조건시유효적。
Mannitol,which is the main component of brown algae,was utilized in this study to produce butanol by Clostridium acetobutylicum (ATCC 824). Plackett-Burman (P-B) and Box-Behnken designs were adopted to optimize the fermentation conditions. Ten factors,including beef extract,yeast extract,tryptone,ammonium acetate,KH2PO4,MgSO4?7H2O,FeSO4?7H2O,size of inoculum, fermentation temperature and initial pH,were considered to affect butanol production in the Plackett-Burman (P-B) design. The result demonstrated that fermentation temperature,initial pH and concentration of ammonium acetate were the most critical factors,and optimal fermentation conditions were 37.3℃,pH 6.38 and 2.82 g/L of ammonium acetate according to the Box-Behnken design. A mathematical model was established and used to predict the maximum butanol yield of 8.47 g/L. The result of verification experiment showed that the maximum butanol yield reached 8.52±0.55g/L under the optimum conditions,which showed that the statistical method was a useful tool for butanol production from mannitol in the optimization.