CAJ | 학술논문

采用原位聚合并表面接枝制备了硅胶衍生尼古丁印迹整体柱。红外光谱和扫描电镜研究了聚合物的结构，基于尺寸排阻色谱原理测定了整体柱的总孔和微孔体积。在高效液相色谱系统中，考察了色谱条件对整体柱选择性的影响；用前沿色谱法测试了 MIP 整体柱的等温吸附，并研究了整体柱的吸附热力学。结果表明： MIP整体柱对尼古丁的选择因子相对8-羟基喹啉和吡啶分别为4.31和2.95；当流动相中 pH 值为7.5时，选择性最好；Freundlich 模型比 Langmuir 模型更适合描述尼古丁在印迹整体柱上表面位点分布，位点异化指数为0.841；吸附热力学分析表明尼古丁在印迹整体柱上的自发放热吸附过程。
채용원위취합병표면접지제비료규효연생니고정인적정체주。홍외광보화소묘전경연구료취합물적결구，기우척촌배조색보원리측정료정체주적총공화미공체적。재고효액상색보계통중，고찰료색보조건대정체주선택성적영향；용전연색보법측시료 MIP 정체주적등온흡부，병연구료정체주적흡부열역학。결과표명： MIP정체주대니고정적선택인자상대8-간기규람화필정분별위4.31화2.95；당류동상중 pH 치위7.5시，선택성최호；Freundlich 모형비 Langmuir 모형경괄합묘술니고정재인적정체주상표면위점분포，위점이화지수위0.841；흡부열역학분석표명니고정재인적정체주상적자발방열흡부과정。
Synthetization of nicotine imprinted polymer (MIP)-derivatized silica monolithic column was carried out based on in situ polymerization on the surface of macroporous silica gel-chemically modified as the matrix. Structural characterization was performed by FTIR,SEM,and the total pores and microspores volume were measured though size exclusion chromatography. In HPLC system, chromatographic condition was tested. Frontal analysis was utilized for producing absorption isotherm for the MIP monolithic column. Additionally,adsorption thermodynamic behavior of the MIP was studied. The result showed that selectivity factors of nicotine were 4.31 and 2.95 relative to 8-hydroxyquinoline and pyridine respectively,and the optimized chromatographic conditions was pH=7.5. Freundlich model was shown better than Langmuir model to describe isotherm adsorption behavior of MIP monolith toward nicotine with a heterogeneity index of 0.841. Thermodynamic analysis indicated a spontaneous exothermic process for the adsorption of template on the MIP monolithic column.