CAJ | 학술논문

建立碱性橙II的酶联免疫分析方法。采用戊二醛偶联法将半抗原碱性橙II与载体牛血清白蛋白（BSA）及卵清蛋白（OVA）制备免疫抗原碱性橙II-BSA和包被抗原碱性橙II-OVA。经紫外可见吸收光谱鉴定和大白兔免疫实验证实人工抗原成功合成，并制备抗碱性橙II的多克隆抗体，该抗体可检测快速定量食品中的碱性橙II，最低检测限为10 pg。该检测方法具有高灵敏度和特异性，可应用于食品中碱性橙II检测试剂盒的研制。
건립감성등II적매련면역분석방법。채용무이철우련법장반항원감성등II여재체우혈청백단백（BSA）급란청단백（OVA）제비면역항원감성등II-BSA화포피항원감성등II-OVA。경자외가견흡수광보감정화대백토면역실험증실인공항원성공합성，병제비항감성등II적다극륭항체，해항체가검측쾌속정량식품중적감성등II，최저검측한위10 pg。해검측방법구유고령민도화특이성，가응용우식품중감성등II검측시제합적연제。
@@@@In order to determined the Chrysoidin II by enzyme-linked immunosorbent assay (ELISA) method, a complete antigen Chrysoidin II -BSA and a coating antigen Chrysoidin II -OVA were prepared by coupling Chrysoidin II with the carrier proteins of bovineserum albumin (BSA)and ovalbumin (OVA) using glutaraldehyde method, respectively. The synthesis of Chrysoidin II artificial antigens was confirmed by UV absorption spectra and animal experiments. A novel anti-Chrysoidin II polyclonal antibody was prepared , which was successfully applied to the determination of Chrysoidin II in food with a limit of detection of 10 pg. High sensitivity and specificity will contribute to the development of commercial ELISA kits for the detection of Chrysoidin II in foods.