CAJ | 학술논문

用木瓜蛋白酶提取林蛙皮胶原蛋白，采用单因素试验和正交试验设计优选最佳提取工艺，并对林蛙皮胶原蛋白的抗氧化性进行研究。结果表明：林蛙皮胶原蛋白的最佳提取工艺是加酶量为1%，料液比为1∶20（g/mL），pH为5.5于室温反应36 h，此条件下胶原蛋白提取率为24.71%，纯度为72.03%。林蛙皮胶原蛋白具有一定的还原能力，对羟自由基具有一定的清除能力，并且随着浓度的增加抗氧化活性逐渐增强。
용목과단백매제취림와피효원단백，채용단인소시험화정교시험설계우선최가제취공예，병대림와피효원단백적항양화성진행연구。결과표명：림와피효원단백적최가제취공예시가매량위1%，료액비위1∶20（g/mL），pH위5.5우실온반응36 h，차조건하효원단백제취솔위24.71%，순도위72.03%。림와피효원단백구유일정적환원능력，대간자유기구유일정적청제능력，병차수착농도적증가항양화활성축점증강。
@@@@Extraction of Rana Chensinensis skin collagen by papain and its antioxidant activity were studied in this paper. The results of the single factor experiment and orthogonal test were as follows: the hydrolysis temperature of room temperature, reaction time of 36 h, pH of 5.5, enzyme concentration of 1%and the ratio of solid to liquid 1∶20 (g/mL). Its recovery yield was 24.71%and the degree of puirty was 72.03%. Collagen of Rana Chensinensis Skin had strong reducing capacity and antioxidant activity on scavenging hydroxyl free radical effects .