食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
10期
9-12
,共4页
肺纤维化%二氧化硅%黑木耳粉%氧化损伤
肺纖維化%二氧化硅%黑木耳粉%氧化損傷
폐섬유화%이양화규%흑목이분%양화손상
pulmonary fibrosis%silica%auricularia auricular powder%oxidative damage
观察黑木耳粉对染矽尘大鼠肺组织纤维化的影响,探讨黑木耳粉对矽肺早期的防治作用。采用非暴露气管插管法给大鼠气管注入二氧化硅(SiO2)粉尘200 mg/kg,染尘后24 h开始以喂饲途径给予黑木耳粉,分别在14、28、56 d时分批处死动物。检测肺组织Ⅰ型胶原纤维、Ⅲ型胶原纤维、超氧化物歧化酶(SOD)、丙二醛(MDA)和血清一氧化氮(NO)含量变化。结果表明:14、28、56 d时,黑木耳粉治疗组大鼠肺组织Ⅰ和Ⅲ型胶原纤维含量与同期SiO2对照组比较有所降低,其中黑木耳粉高剂量组大鼠肺组织Ⅰ和Ⅲ型胶原纤维含量与同期SiO2对照组比较,差异均有显著性(P<0.05);黑木耳粉治疗组大鼠肺组织SOD活性高于同期SiO2对照组,MDA含量低于同期SiO2对照组,且在14 d时,黑木耳粉各个剂量组SOD活性与SiO2对照组比较,差异均有显著性(P<0.05);黑木耳粉治疗组大鼠血清NO含量均低于同期SiO2对照组,黑木耳粉高剂量组与同期SiO2对照组比较差异有显著性(P<0.05)。黑木耳粉能够减轻染石英尘大鼠肺组织氧化损伤,可降低肺组织胶原纤维含量,提示其对矽肺纤维化具有一定的防治作用。
觀察黑木耳粉對染矽塵大鼠肺組織纖維化的影響,探討黑木耳粉對矽肺早期的防治作用。採用非暴露氣管插管法給大鼠氣管註入二氧化硅(SiO2)粉塵200 mg/kg,染塵後24 h開始以餵飼途徑給予黑木耳粉,分彆在14、28、56 d時分批處死動物。檢測肺組織Ⅰ型膠原纖維、Ⅲ型膠原纖維、超氧化物歧化酶(SOD)、丙二醛(MDA)和血清一氧化氮(NO)含量變化。結果錶明:14、28、56 d時,黑木耳粉治療組大鼠肺組織Ⅰ和Ⅲ型膠原纖維含量與同期SiO2對照組比較有所降低,其中黑木耳粉高劑量組大鼠肺組織Ⅰ和Ⅲ型膠原纖維含量與同期SiO2對照組比較,差異均有顯著性(P<0.05);黑木耳粉治療組大鼠肺組織SOD活性高于同期SiO2對照組,MDA含量低于同期SiO2對照組,且在14 d時,黑木耳粉各箇劑量組SOD活性與SiO2對照組比較,差異均有顯著性(P<0.05);黑木耳粉治療組大鼠血清NO含量均低于同期SiO2對照組,黑木耳粉高劑量組與同期SiO2對照組比較差異有顯著性(P<0.05)。黑木耳粉能夠減輕染石英塵大鼠肺組織氧化損傷,可降低肺組織膠原纖維含量,提示其對矽肺纖維化具有一定的防治作用。
관찰흑목이분대염석진대서폐조직섬유화적영향,탐토흑목이분대석폐조기적방치작용。채용비폭로기관삽관법급대서기관주입이양화규(SiO2)분진200 mg/kg,염진후24 h개시이위사도경급여흑목이분,분별재14、28、56 d시분비처사동물。검측폐조직Ⅰ형효원섬유、Ⅲ형효원섬유、초양화물기화매(SOD)、병이철(MDA)화혈청일양화담(NO)함량변화。결과표명:14、28、56 d시,흑목이분치료조대서폐조직Ⅰ화Ⅲ형효원섬유함량여동기SiO2대조조비교유소강저,기중흑목이분고제량조대서폐조직Ⅰ화Ⅲ형효원섬유함량여동기SiO2대조조비교,차이균유현저성(P<0.05);흑목이분치료조대서폐조직SOD활성고우동기SiO2대조조,MDA함량저우동기SiO2대조조,차재14 d시,흑목이분각개제량조SOD활성여SiO2대조조비교,차이균유현저성(P<0.05);흑목이분치료조대서혈청NO함량균저우동기SiO2대조조,흑목이분고제량조여동기SiO2대조조비교차이유현저성(P<0.05)。흑목이분능구감경염석영진대서폐조직양화손상,가강저폐조직효원섬유함량,제시기대석폐섬유화구유일정적방치작용。
@@@@To study the effect of auricularia auricular powder (AAP) on pulmonary fibrosis to find an effective treatment on early-stage salvia . Silicosis models were established by direct tracheal instillation of silica 200 mg/kg into rat lungs non-surgically. After 24 hours,rats were given AAP by feeding. At 14th,28th and 56th day, eight rats were killed in a group at a time. Ⅰfibrosis content , Ⅲ fibrosis content, the superoxide dismutase (SOD)activities,maleic dialdehyde (MDA) content and glutathione peroxidase (NO) content in the serum were assayed. At 14th,28th and 56th day,ⅠandⅢfibrosis content of AAP-treated groups were lower than those of contemporaneous SiO2 control group , and Ⅰand Ⅲ fibrosis content were significantly difference between the AAP-treated groupⅢand SiO2 control group (P<0.05).SOD activities were higher than those of contemporaneous SiO2 control group,and MDA content were lower than those of contemporaneous SiO2 control group, moreover,at 14 th day, SOD activities of AAP-treated groups were significantly higher than those of SiO2 control group (P <0.05). serum NO content of AAP -treated groups were lower than those of contemporaneous SiO2 control group,and serum NO content were significantly difference between the AAP-treated groupⅢand SiO2 control group(P<0.05). AAP can resist the oxidative damage and inhibit synthesization of lung collagen fibrosis to prevention silicotic fibrosis.