食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
11期
57-60
,共4页
液质联用仪%潮汕猪肉丸%β-受体激动剂%盐酸克伦特罗%莱克多巴胺
液質聯用儀%潮汕豬肉汍%β-受體激動劑%鹽痠剋倫特囉%萊剋多巴胺
액질련용의%조산저육환%β-수체격동제%염산극륜특라%래극다파알
HPLC/MS%chaoshan pork balls%β-agonists%clenbuterol%ractopamine
通过对样品中待测组分的提取、净化，建立一种快速测定潮汕猪肉丸中盐酸克伦特罗和莱克多巴胺两种β-受体激动剂残留量的液质联用检测方法。样品用β-葡萄糖醛苷酸酶溶液酶解，20 mmol/L乙酸铵缓冲溶液（pH5.2）提取，固相萃取柱净化，通过质谱对样品进行确证，外标峰面积法定量。方法加标平均回收率盐酸克伦特罗为98.6%～110.1%，试验的RSD为0.26%～0.93%；莱克多巴胺为90.1%～101.3%，试验的RSD为0.22%～1.33%。该方法优化了盐酸克伦特罗和莱克多巴胺的仪器分析条件，操作方便，重现性好，回收率高，结果准确，能满足食品安全控制的检测需要。
通過對樣品中待測組分的提取、淨化，建立一種快速測定潮汕豬肉汍中鹽痠剋倫特囉和萊剋多巴胺兩種β-受體激動劑殘留量的液質聯用檢測方法。樣品用β-葡萄糖醛苷痠酶溶液酶解，20 mmol/L乙痠銨緩遲溶液（pH5.2）提取，固相萃取柱淨化，通過質譜對樣品進行確證，外標峰麵積法定量。方法加標平均迴收率鹽痠剋倫特囉為98.6%～110.1%，試驗的RSD為0.26%～0.93%；萊剋多巴胺為90.1%～101.3%，試驗的RSD為0.22%～1.33%。該方法優化瞭鹽痠剋倫特囉和萊剋多巴胺的儀器分析條件，操作方便，重現性好，迴收率高，結果準確，能滿足食品安全控製的檢測需要。
통과대양품중대측조분적제취、정화，건립일충쾌속측정조산저육환중염산극륜특라화래극다파알량충β-수체격동제잔류량적액질련용검측방법。양품용β-포도당철감산매용액매해，20 mmol/L을산안완충용액（pH5.2）제취，고상췌취주정화，통과질보대양품진행학증，외표봉면적법정량。방법가표평균회수솔염산극륜특라위98.6%～110.1%，시험적RSD위0.26%～0.93%；래극다파알위90.1%～101.3%，시험적RSD위0.22%～1.33%。해방법우화료염산극륜특라화래극다파알적의기분석조건，조작방편，중현성호，회수솔고，결과준학，능만족식품안전공제적검측수요。
@@@@A method was developed for fhe determination of clenbuterol and ractopamine residues in Chaoshan pork balls by SPE-LC-MS/MS. The sample was extracted and purified by solid phase extraction(SPE)cartridge, analyzed by LC-MS/MS.The sample was hydrolyzed by β-glucuronic acid enzyme,extracted by ammonium acetate buffer solution (pH5.2)and purified by solid phase extraction (SPE)cartridge.finally,confirmed by mass spectrometry and the quantity is set by external standard peak area.The rate of average recovery of clenbuterol was 98.6%-110.1%,and RSDs ranged from 0.26%to 0.93%;ractopamine was 90.1%-101.3%,and RSDs ranged from 0.22 % to 1.33 %.In this paper,optimized the condition of LC-MS/MS,the method has the advantages of simplicity,rapidity,precision,good reproducibility and high recovery,so that it could be used for the determination of clenbuterol and ractopamine residues ,and it can meet the need of measuring in food safety control.
