食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
12期
93-96
,共4页
王雪莹%陆舍铭%朱丽%孟昭宇%曹秋娥
王雪瑩%陸捨銘%硃麗%孟昭宇%曹鞦娥
왕설형%륙사명%주려%맹소우%조추아
超高效液相色谱法%香精%可可碱
超高效液相色譜法%香精%可可堿
초고효액상색보법%향정%가가감
UPLC%flavor%theobromine
建立了测定香精中可可碱的超高效液相色谱方法。以超纯水为萃取剂，采用ACQUITY UPLC R BEH C18（2.1 mm i.d×50 mm，1.7滋m）；柱温为30℃；流动相为甲醇-水，进行梯度洗脱；流速为0.1 mL/min；检测波长为274 nm，对20种香精样品进行测定。结果表明：可可碱标准品的色谱峰峰型尖锐且对称；在0.16 mg/L~8.00 mg/L范围内呈良好线性关系（r=0.9998）；可可碱相对标准偏差在0.14%～1.58%之间，平均回收率在91.86%～93.70%之间。
建立瞭測定香精中可可堿的超高效液相色譜方法。以超純水為萃取劑，採用ACQUITY UPLC R BEH C18（2.1 mm i.d×50 mm，1.7滋m）；柱溫為30℃；流動相為甲醇-水，進行梯度洗脫；流速為0.1 mL/min；檢測波長為274 nm，對20種香精樣品進行測定。結果錶明：可可堿標準品的色譜峰峰型尖銳且對稱；在0.16 mg/L~8.00 mg/L範圍內呈良好線性關繫（r=0.9998）；可可堿相對標準偏差在0.14%～1.58%之間，平均迴收率在91.86%～93.70%之間。
건립료측정향정중가가감적초고효액상색보방법。이초순수위췌취제，채용ACQUITY UPLC R BEH C18（2.1 mm i.d×50 mm，1.7자m）；주온위30℃；류동상위갑순-수，진행제도세탈；류속위0.1 mL/min；검측파장위274 nm，대20충향정양품진행측정。결과표명：가가감표준품적색보봉봉형첨예차대칭；재0.16 mg/L~8.00 mg/L범위내정량호선성관계（r=0.9998）；가가감상대표준편차재0.14%～1.58%지간，평균회수솔재91.86%～93.70%지간。
@@@@To establish a method for Simultaneous determination of theobromine in tobacco flavor by reversed phase ultra performance liquid chromatography.20 kinds of tobacco flavor samples were extracted by water separated at 30℃on ACQUITY UPLC RBEH C18 (2.1 mm i.d×50 mm,1.7μm) column eluted with methanol-water as the mobile phase .Flow rate was set at 0.1 mL/min and the detection wavelength was set at 274 nm. The results indicated that:The chromatographic peak of theobromine standard samples were sharp and symmetric. The calibration curve of theobromine was linear within the range from 0.16 to 8.00 mg/L(r=0.999 8). The RSD of theobromine were from 0.14 %to 1.58 %in three days,and the average recoveries of theobromine were from 91.86 % to 93.70 %.
