CAJ | 학술논문

选取哺乳动物牛的喉管软骨为实验材料，用酶法提取多糖并运用响应面法对其提取工艺中温度、pH、搅拌时间进行了优化分析，得到的最佳工艺条件为温度53.41℃、pH8.23连续搅拌酶解9.33 h；此条件下的多糖提取率为20.05%，酸性多糖含量达39.58%。通过高效液相色谱法，初步断定软骨多糖为类硫酸软骨素产品。
선취포유동물우적후관연골위실험재료，용매법제취다당병운용향응면법대기제취공예중온도、pH、교반시간진행료우화분석，득도적최가공예조건위온도53.41℃、pH8.23련속교반매해9.33 h；차조건하적다당제취솔위20.05%，산성다당함량체39.58%。통과고효액상색보법，초보단정연골다당위류류산연골소산품。
@@@@In order to study the extraction process of cartilage polysaocharide ,the costal cartilage of bovine was selecIed as materials and the enzymatic processing technology for polysaccharide was studied in this paper. Through Response Surface Methodology, the optimum process parameters were extracted for 9.33 h at pH8.23 and 53.41℃. Under this optimal condition, the extraction rates was 20.05%and the content of polysaccharides was 39.58 %. Through High Performance Liquid Chromatography , the Cartilage polysaccharides was were determined as the similar of chondroitin sulfate.