CAJ | 학술논문

目的通过应用免疫组化方法研究胃癌患者原发灶转移及邻近非肿瘤胃黏膜等不同组织的基质金属蛋白酶-2（MMP2）和整合素β1亚单位分布特征，旨在探讨整合素β1、MMP2的表达与胃癌侵袭性的关系，并指导临床治疗及判断胃癌患者的预后。方法采用免疫组织化学通用二步法检测整合素β1和MMP2在64例胃癌原发灶及其淋巴结转移灶中的表达情况。结果整合素β1在正常胃小凹表层上皮细胞膜表面呈均质性表达，并沿基底膜呈连续性线状分布；在胃癌组织的细胞浆和细胞膜呈棕黄色表达；淋巴结转移灶中呈非均质性棕黄色表达。在胃癌组织中的阳性表达率明显高于邻近的正常黏膜组织（P＜0.05）；在不同的组织学类型整合素β1阳性表达也有差异，在高中分化组为40%，在低分化组中为84.6%，可见，在低分化癌明显高于高中分化癌（P＜0.01）；整合素β1的阳性表达率与患者的年龄、性别、肿瘤的大小无明显相关性（P＞0.05）。胃癌组织中MMP2阳性主要表达于胃癌组织的细胞浆和细胞膜内，呈棕黄色。MMP2表达阳性在胃癌组织明显强于邻近正常黏膜组织（P＜0.05）；浸透黏膜层组中的表达强于未浸透组（P＜0.05）；有淋巴结转移组的阳性表达高于无淋巴结转移组；不同的组织学类型中阳性表达也有差异，在低分化癌明显高于高中分化癌（P＜0.01）。MMP2在胃癌中的表达与性别、年龄、肿瘤大小无关。结论整合素β1和MMP2的表达与胃癌的分化程度、侵润程度、淋巴结转移状况等临床病理因素密切相关，而与年龄、性别、肿瘤的大小等无关，其表达的检测对评估胃癌淋巴结转移程度有重要的意义，还可以作为估计患者预后及术后治疗的生物学指标。
목적통과응용면역조화방법연구위암환자원발조전이급린근비종류위점막등불동조직적기질금속단백매-2（MMP2）화정합소β1아단위분포특정，지재탐토정합소β1、MMP2적표체여위암침습성적관계，병지도림상치료급판단위암환자적예후。방법채용면역조직화학통용이보법검측정합소β1화MMP2재64례위암원발조급기림파결전이조중적표체정황。결과정합소β1재정상위소요표층상피세포막표면정균질성표체，병연기저막정련속성선상분포；재위암조직적세포장화세포막정종황색표체；림파결전이조중정비균질성종황색표체。재위암조직중적양성표체솔명현고우린근적정상점막조직（P＜0.05）；재불동적조직학류형정합소β1양성표체야유차이，재고중분화조위40%，재저분화조중위84.6%，가견，재저분화암명현고우고중분화암（P＜0.01）；정합소β1적양성표체솔여환자적년령、성별、종류적대소무명현상관성（P＞0.05）。위암조직중MMP2양성주요표체우위암조직적세포장화세포막내，정종황색。MMP2표체양성재위암조직명현강우린근정상점막조직（P＜0.05）；침투점막층조중적표체강우미침투조（P＜0.05）；유림파결전이조적양성표체고우무림파결전이조；불동적조직학류형중양성표체야유차이，재저분화암명현고우고중분화암（P＜0.01）。MMP2재위암중적표체여성별、년령、종류대소무관。결론정합소β1화MMP2적표체여위암적분화정도、침윤정도、림파결전이상황등림상병리인소밀절상관，이여년령、성별、종류적대소등무관，기표체적검측대평고위암림파결전이정도유중요적의의，환가이작위고계환자예후급술후치료적생물학지표。
Objective But the report about this study in gastric carcinoma is still little. This experiment aims to study the expression if integrinsβ1 and MMP2 cell adhesion receptors and the relationship between it and the affection of gastric carcinoma. Method Immunohistochemistry method was used to examine integrinsβ1 and MMP2 in gastric carcinoma and its lymphatic metastasis. Results Intergrinβ1 is mainly expressed in gastric tissues and the metastasis lymphatic nodes, but hardly expressed in normal tissues.The positive expression of intergrinβ1 is localized in the cancerous cytoplasm and membranes.it is significantly in the gastric tumor group than in normal tissues.In carcinoma guoup, integrinβ1, s expression is associated with factors such as differentiation degree, invasion deep, lymphatic and peritoneal metastasis. MMP2 is mainly expressed in the cancerous cytoplasm and membrane, it is significantly in the gastric tumor group than in normal.the expression was highly related with lymphatic metastasis, differentiation and invasion degree, and lowly related with age, sex and so on. Conclusion The expression of integrinsβ1 and MMP2 was closely related to clinical factors of gastric carcinoma, such as the degree of differentiation and invasion, metastasis of lymph modes and liver.It may be useful to evaluate the degree of lymph metastasis in gastric carcinoma and estimate sufferer prognosis by detecting expression of integrinβ1 and MMP2 in gastric carcinoma and its lymph nodes.