中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
14期
403-404,405
,共3页
甘露茶%防风%广藿香%山楂%鸭脚木叶和青蒿药材%橙皮苷%显微鉴别%高效液相色谱法
甘露茶%防風%廣藿香%山楂%鴨腳木葉和青蒿藥材%橙皮苷%顯微鑒彆%高效液相色譜法
감로다%방풍%엄곽향%산사%압각목협화청호약재%등피감%현미감별%고효액상색보법
Ganlucha%Saposhnikoviae Radix%Pogostemonis Herba%Crataegi Fructus%Sehefflera octophylla%Artemisiae Annuae Herba%Hesperidi%Microscopical identification%HPLC
目的建立甘露茶定性、定量的检验方法，其目标为显微鉴别和高效液相含量测定。方法采用中药材粉末显微鉴定，寻找甘露茶在显微镜下的防风、广藿香、山楂、鸭脚木叶和青蒿药材的特征性细胞；采用高效液相法测定甘露茶中青皮药材的含量，流动相：乙腈-0.2%磷酸溶液（20∶80）；检测波长：284nm；流速：1.0mL/min；柱温：35℃。结果显微鉴定：防风、广藿香、山楂、鸭脚木叶和青蒿药材特征性强具有一定的代表性；含量测定：青皮药材中橙皮苷在7.5188~481.2μg 之间呈良好的线性关系，回归方程为 Y=17.239x ＋17.608， R2=1（n=7），平均回收率为99.87%，RSD=1.94%（n=6）。结论本方法快速、简便、准确，专属性强，重现性好，可作为甘露茶质量控制的方法。
目的建立甘露茶定性、定量的檢驗方法，其目標為顯微鑒彆和高效液相含量測定。方法採用中藥材粉末顯微鑒定，尋找甘露茶在顯微鏡下的防風、廣藿香、山楂、鴨腳木葉和青蒿藥材的特徵性細胞；採用高效液相法測定甘露茶中青皮藥材的含量，流動相：乙腈-0.2%燐痠溶液（20∶80）；檢測波長：284nm；流速：1.0mL/min；柱溫：35℃。結果顯微鑒定：防風、廣藿香、山楂、鴨腳木葉和青蒿藥材特徵性彊具有一定的代錶性；含量測定：青皮藥材中橙皮苷在7.5188~481.2μg 之間呈良好的線性關繫，迴歸方程為 Y=17.239x ＋17.608， R2=1（n=7），平均迴收率為99.87%，RSD=1.94%（n=6）。結論本方法快速、簡便、準確，專屬性彊，重現性好，可作為甘露茶質量控製的方法。
목적건립감로다정성、정량적검험방법，기목표위현미감별화고효액상함량측정。방법채용중약재분말현미감정，심조감로다재현미경하적방풍、엄곽향、산사、압각목협화청호약재적특정성세포；채용고효액상법측정감로다중청피약재적함량，류동상：을정-0.2%린산용액（20∶80）；검측파장：284nm；류속：1.0mL/min；주온：35℃。결과현미감정：방풍、엄곽향、산사、압각목협화청호약재특정성강구유일정적대표성；함량측정：청피약재중등피감재7.5188~481.2μg 지간정량호적선성관계，회귀방정위 Y=17.239x ＋17.608， R2=1（n=7），평균회수솔위99.87%，RSD=1.94%（n=6）。결론본방법쾌속、간편、준학，전속성강，중현성호，가작위감로다질량공제적방법。
Objective In order to search an easy ,accurate and quick method to control the quality of Ganlucha, we establish a microscopical identification for the characteristic identification and a HPLC method for the content determination of Ganlucha. Method We use the Chinese herbal medicine powder microscopical identification method to identify the characteristic cells of the Saposhnikoviae Radix, the Pogostemonis Herba, the Crataegi Fructus, the Sehefflera octophylla and the Artemisiae Annuae Herba. We use the HPLC method to determine the content of the Pericarpium citri reticulatae viride. Mobile phase: Acetonitrile - 0.2% phosphoric acid (20∶80); detection wavelength: 284 nm; Flow rate: 1.0mL/min; Column temperature 35℃. Results Microscopical identification: The Saposhnikoviae Radix, the Pogostemonis Herba, the Crataegi Fructus, the Sehefflera octophylla and the Artemisiae Annuae Herba show the strong characters; HPLC: the result shows the method is accurate and easy to handle. In the range of 7.5188-481.2μg, the Hesperidi in the Pericarpium Citri Reticulatae Viride herbal shows a good linear relationship. The regression equation is Y=17.239 x +17.608, R2=1 (n=7) and the average recovery is 99.87%, RSD=1.94% (n=6). Conclusion The identification of the Saposhnikoviae Radix, the Pogostemonis Herba, the Crataegi Fructus, the Sehefflera octophylla and the Artemisiae Annuae Herba. by microscopical identification method is accurate and easy. The content determination of the Hesperidi by HPLC is simple, specific and reproducible. We can use these methods to control the quality of Ganlucha.
