CAJ | 학술논문

目的评价454 GS Junior 测序平台在细菌基因组测序中的应用价值。方法利用454 GS Junior 测序平台测定1株霍乱弧菌的基因组序列，组装注释后，与参考序列霍乱弧菌 O1埃尔托生物型菌株 N16961基因组序列进行比对。结果共获得37Mbp 数据，平均读长为322bp。数据组装后，得到170个序列重叠群，总长为3.96Mbp。与参考序列相比，测定序列对于参考序列的覆盖度达到99.49%，参考序列96.76%的基因被完整测出同源基因。结论454 GS Junior 测序平台可以用于细菌基因组的快速测定。
목적평개454 GS Junior 측서평태재세균기인조측서중적응용개치。방법이용454 GS Junior 측서평태측정1주곽란호균적기인조서렬，조장주석후，여삼고서렬곽란호균 O1애이탁생물형균주 N16961기인조서렬진행비대。결과공획득37Mbp 수거，평균독장위322bp。수거조장후，득도170개서렬중첩군，총장위3.96Mbp。여삼고서렬상비，측정서렬대우삼고서렬적복개도체도99.49%，삼고서렬96.76%적기인피완정측출동원기인。결론454 GS Junior 측서평태가이용우세균기인조적쾌속측정。
Objective To investigate the application value of 454 GS Junior Sequencing Platform in bacterial genome sequencing. Methods Genome sequencing of Vibrio Cholera was performed using 454 pyrosequencing, and the genome was compared with the reference sequence of N16961 after assembling and annotation. Results Totally, 37 Mbp raw data were obtained and the average reads length is 322 bps. 170 contigs were assembled and the whole genome is 3.96 Mbp. Comparing with strain N16961, the sequenced genome covered 99.49% of the reference genome and 96.76% genes of N16961 got orthologous in the sequenced genome. Conclusion The 454 GS Junior Sequencing Platform is suitable for the fast sequencing of bacterial genome.