CAJ | 학술논문

以栽培番茄1052为母本，以秘鲁番茄LA3858为父本进行杂交，通过幼胚挽救培养获得了1株F1植株，再以栽培番茄1052为母本进行回交，通过幼胚挽救获得了6株BC1单株，利用129个分布于12条染色体上的CAPS、SSR和InDel标记对BC1单株进行染色体片段分析，结果表明6株BC1单株中12条染色体含有的秘鲁番茄片段涵盖了秘鲁番茄LA3858的整个基因组。
이재배번가1052위모본，이비로번가LA3858위부본진행잡교，통과유배만구배양획득료1주F1식주，재이재배번가1052위모본진행회교，통과유배만구획득료6주BC1단주，이용129개분포우12조염색체상적CAPS、SSR화InDel표기대BC1단주진행염색체편단분석，결과표명6주BC1단주중12조염색체함유적비로번가편단함개료비로번가LA3858적정개기인조。
In this paper,cultivated tomato Lycopersicon esculentum Mill. 1052 was used as female parent and wild tomato L. peruvianum LA3858 was used as male parent.One F1 hybrid was obtained by embryo rescuing method.The hybrid was backcrossed with Lycopersicon esculentum Mill. 1052 to generate BC1 progeny.And 6 single plants of backcross F1 species hybrids were obtained. Moreover,129 primers of CAPS,SSR and InDel on 12 chromosomes were used to analyze chromosome fragments of BC1. The results showed that these 6 BC1 plants contained the whole genome of L. peruvianum LA3858.