中国蔬菜
中國蔬菜
중국소채
CHINA VEGETABLES
2013年
14期
25-30
,共6页
王兆吉%高鹏%栾非时%刘识
王兆吉%高鵬%欒非時%劉識
왕조길%고붕%란비시%류식
西瓜%果形指数%SSR标记%QTL分析
西瓜%果形指數%SSR標記%QTL分析
서과%과형지수%SSR표기%QTL분석
Watermelon%Fruit shape index%SSR marker%QTL analysis
以圆球形西瓜品系花园母本为母本,椭圆形西瓜品系LSW-177为父本,配制杂交组合,构建由180个单株组成的F2群体,测量成熟西瓜的果实纵径、果实横径,并且通过果实的纵径与横径的比值得到果形指数,进行遗传图谱的构建及相关性状QTL分析。构建的连锁遗传图谱共含有45个SSR标记,分属13个连锁群。覆盖基因组长度为547.3 cM,标记间平均距离为12.44 cM,检测到与果形指数密切相关的QTL 1个,并在遗传连锁图谱中将之命名为Fsi3.1,位于第三连锁群,位于标记MCPI-12和CMN05_69之间,贡献率为2.8%,加性效应负值,对减小果形指数表现为增效加性效应。
以圓毬形西瓜品繫花園母本為母本,橢圓形西瓜品繫LSW-177為父本,配製雜交組閤,構建由180箇單株組成的F2群體,測量成熟西瓜的果實縱徑、果實橫徑,併且通過果實的縱徑與橫徑的比值得到果形指數,進行遺傳圖譜的構建及相關性狀QTL分析。構建的連鎖遺傳圖譜共含有45箇SSR標記,分屬13箇連鎖群。覆蓋基因組長度為547.3 cM,標記間平均距離為12.44 cM,檢測到與果形指數密切相關的QTL 1箇,併在遺傳連鎖圖譜中將之命名為Fsi3.1,位于第三連鎖群,位于標記MCPI-12和CMN05_69之間,貢獻率為2.8%,加性效應負值,對減小果形指數錶現為增效加性效應。
이원구형서과품계화완모본위모본,타원형서과품계LSW-177위부본,배제잡교조합,구건유180개단주조성적F2군체,측량성숙서과적과실종경、과실횡경,병차통과과실적종경여횡경적비치득도과형지수,진행유전도보적구건급상관성상QTL분석。구건적련쇄유전도보공함유45개SSR표기,분속13개련쇄군。복개기인조장도위547.3 cM,표기간평균거리위12.44 cM,검측도여과형지수밀절상관적QTL 1개,병재유전련쇄도보중장지명명위Fsi3.1,위우제삼련쇄군,위우표기MCPI-12화CMN05_69지간,공헌솔위2.8%,가성효응부치,대감소과형지수표현위증효가성효응。
Taking round shape watermelon line‘Huayuanmuben’as female parent and oval shape line‘LSW-177’as male parent,we crossed them and obtained F2 population constructed with 180 single plants.We measured the length and width of the ripened watermelon〔Citrullus lanatus (Thunb.)Matsum. et Nakai〕,obtained fruit shape index from the ratio between fruit length and width, constructed the genetic map and analyzed the QTLs of relevant characteristics.The constructed genetic map contains 45 SSR-markers belonging to 13 linkage groups.The covering genome length was 547.3 cM.The average distance between markers was 12.44 cM.The one QTL closely associated with fruit shape index was located in the 3rd linkage group between MCPI-12 and CMN05_69.This QTL was named as Fsi3.1.Its contribution rate was 2.8%,and its additive effect was negative,which showed synergia additive effect in reducing fruit shape index.